Antioxidant activity versus cytotoxic and NF-κ B regulatory activities on HT-29 cells by natural fruit juices

Kulišić-Bilušić, Tea; Schnäbele, Kerstin; Schmöller, Ingrid; Dragović-Uzelac, Verica; Kriško, Anita; Dejanović, Branka; Miloš, Mladen; Pifat, Greta

doi:10.1007/s00217-008-0948-1

Pregled bibliografske jedinice broj: 370310

Antioxidant activity versus cytotoxic and NF-κ B regulatory activities on HT-29 cells by natural fruit juices

Kulišić-Bilušić, Tea; Schnäbele, Kerstin; Schmöller, Ingrid; Dragović-Uzelac, Verica; Kriško, Anita; Dejanović, Branka; Miloš, Mladen; Pifat, Greta

Antioxidant activity versus cytotoxic and NF-κ B regulatory activities on HT-29 cells by natural fruit juices // European food research and technology, 228 (2009), 3; 417-424 doi:10.1007/s00217-008-0948-1 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 370310 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Antioxidant activity versus cytotoxic and NF-κ B regulatory activities on HT-29 cells by natural fruit juices

Autori
Kulišić-Bilušić, Tea ; Schnäbele, Kerstin ; Schmöller, Ingrid ; Dragović-Uzelac, Verica ; Kriško, Anita ; Dejanović, Branka ; Miloš, Mladen ; Pifat, Greta

Izvornik
European food research and technology (1438-2377) 228 (2009), 3; 417-424

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Fruit juices phenols ; Antioxidant activity ; Free radical scavenging ; T 29H

Sažetak
The antioxidant activity (free radical scavenging ability and lipid oxidation inhibitory activity) and the activity in cell culture (influence on HT-29 human colon cancer cell viability and NF-κ B activation) of natural fruit juices from red grape, strawberry, cherry and sour cherry were investigated in vitro. Analysis of polyphenolic composition of fruit juices showed the dominant presence of anthocyanins (cyanidin-3-glucoside, cyanidin-3-rutinoside, peonidin-3-rutinoside, pelargonidin-3-glucoside), anthocyanidins (procyanidins B1, B2 and B3), flavonoids (glucosides of myricetin, quercetin and kaempferol), and phenolic acids (neochlorogenic acid, chlorogenic acid, ellagic acid, p-coumaroylquinic acid) in juices. DPPH test and β -carotene bleaching method indicated that grape, strawberry, cherry and sour cherry natural juices (at the concentration of 2.5%) showed very strong free radical scavenging (75-92%) and antioxidant activity (71-94%). In addition, at the concentration of 0.01% they showed complete inhibition of copper-induced oxidation of human LDL (low density lipoprotein). In contrast, selected fruit juices did not show cytotoxic effects on HT-29 cells and NF-κ B inhibitory activity (at the concentrations of 0.5% and 2%). Results of this study showed no correlation between the antioxidant activity of natural fruit juices from red grape, strawberry, cherry and sour cherry and their effect on HT-29 cell viability and NF-κ B inhibitory activity.

Izvorni jezik
Engleski

Znanstvena područja
Prehrambena tehnologija

POVEZANOST RADA

Projekti:
011-2160547-1330 - Antioksidacijski sastojci i inhibitori kolinesteraza iz aromatičnog bilja (Miloš, Mladen, MZOS ) ( CroRIS)

Ustanove:
Kemijsko-tehnološki fakultet, Split,
Institut "Ruđer Bošković", Zagreb

Profili:

Anita Kriško (autor)