Naslov
Chromosome segregation and cell morphology in recBC sbcBC ruvC mutants of Escherichia coli

Autori
Zahradka, Davor ; Vlahović, Ksenija ; Petranović, Mirjana ; Petranović, Drago

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the 26th Meeting of the Federation of European Biochemical Societies (FEBS'99) ; u: Biochimie 81 (1999) (6/S1) / - Pariz : Elsevier, 1999

Skup
Meeting of the Federation of European Biochemical Societies (26 ; 1999)

Mjesto i datum
Nica, Francuska, 19.06.1999. - 24.06.1999

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Sažetak
The RuvC protein is important for DNA recombination and recombinational repair in E. coli. Together with RuvA and RuvB proteins, it is involved in the final, postsynaptic stage of recombination, in which Holliday intermediates are processed into mature recombinant products. In this work we have been focused on cytological effects provoked by a ruvC mutation in both wild type and recBC sbcBC backgrounds. By the usage of phase-contrast and fluorescence microscopy we have found that the ruvC mutation causes chromosome segregation and cell division defects in exponentially growing cells. These defects were extremely pronounced in recBC sbcBC ruvC mutants, in which about 50 % of population consisted of long filaments with unsegregated chromosomes. A small proportion of filaments displayed unusual shape irregularities including bulges and lateral appendages. Both chromosome segregation and cell division were significantly improved when additional recA mutation was introduced into the ruvC mutants. From these results we infer that chromosome segregation and cell division defects result from the abortive recombination initiated by the RecA protein. We hypothesize that unresolved chromosomes exert a negative effect on cell division process and that they affect cell wall structure.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA