аЯрЁБс>ўџ 46ўџџџ3џџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџьЅС @ јП*bjbjКїКї ."ии* џџџџџџˆbbbbbbbv^^^^$‚v№ ЖЂИИИИИИИ“ • • • • • • І Rј6• b!ИИ!!• bbИИЊ у у у !DbИbИ“ у !“ у у bbу И– ТЗЬЩќШ^e ^у “ Р 0№ у .У .у vvbbbbу .bї œИh Jу j<І{ИИИ• • vvЄDй vvEffect of Grapevine Solarization Technique on the Anthocyanin Content of Autochthonous Croatian Red Wine Teran (Vitis vinifera L.) Maslov, L.*, Karoglan, M., Kozina, B. Department of Viticulture and Enology, Faculty of Agriculture, University of Zagreb, Svetosimunska 25, 10 000 Zagreb, Croatia Abstract The main purpose of grapevine solarization technique is reflection of sunlight into the grapes, and therefore changes in light spectrum and temperature can be noticed. An artificial solarization experiment was conducted on a parcel of Teran grapevines grown in the subregion Istra (Brtonigla vineyard location) of Croatia in the year 2005 and 2006. Main organic acids (tartaric, malic, lactic, citric and succinic) were analysed by HPLC using DAD set at 210 nm on C-18 analytical column. Samples were prepared by solid phase extraction using SAX cartridges in order to clean up interferences like sugars and alcohols. It is well known that Teran is one of the red grape varieties characterized by high to very high total acidity (8,0-14,0 g/L) and high content of malic acid (4,0-10,0 g/L). Compared with standard vinestocks grown on bare soil, solarization technique also caused higher anthocyanins content in Teran wines, analysed by HPLC method just after the end of alcoholic fermentation. A simple and fast method was used for separation of nine anthocyanins in Teran wine by direct injection using DAD set at 518 nm. Phosphate buffer at pH 1.6 and acetonitrile were used as mobile phases. The low pH of the mobile phase is required to keep the anthocyanins homogeneous in the flavylium form according to the pH-dependent equilibria. The linearity was investigated for malvidine-3- glucoside in the range of 5-250 mg/L at seven concentration levels. All of the samples exhibited malvidin-3-monoglucoside as the major compound, representing about 55% of the sum of all individual anthocyanins. The strongest solarization effect was seen through the higher content of 3-O-glucosides of delphinidine, petunidin and malvidin and acetylated as well as coumarylated forms of malvidine and peonidin. Total phenolic contents of samples were also determined using the Folin-Ciocalteu method. The wines were sensory tested by the O.I.V./U.I.OE. method by 100 positive points. This study suggested that solarization technique positively influenced the quality of Teran wines. Key words: wine, solarization, Teran, anthocyanins, HPLC *Corresponding author. Tel.: + 385 1 2343 657; fax: + 385 1 2301 188 E-mail address:  HYPERLINK "mailto:lmaslov@agr.hr" lmaslov@agr.hr (Luna Maslov) Ipƒ„ŽЉЊЋщъ   ) * + , 5 6 яуеЪЛЉЛš‹ƒxpƒhƒ`UNA4hД Ь5CJaJmHsHhД Ь5CJaJmHsH hД-пhД ЬhД-пhJSCJaJhN3‹CJaJh4WЧCJaJh2,CJaJh2,h2,CJaJhД ЬCJaJhђЂh‘BšCJaJmHsHhN3‹hД ЬCJaJmHsH"hN3‹hˆd5CJH*aJmHsHhN3‹h‘BšCJaJmHsHhˆdhмбCJaJhN3‹hД Ь56CJaJhN3‹hД Ь5CJaJhN3‹hД Ь5CJaJmHsHƒ„ЊЋ) * + , 5 6 PQŠ‹ямддШМАЋЂЂ••†}„6]„6gdN3‹$„ўdh]„ўa$gdД Ь $„Х*$`„Хa$gd’V„ў]„ўgdД ЬgdД Ь $„6]„6a$gdД-п $„6]„6a$gdN3‹ $„6]„6a$gdД-п$a$gd‘Bš$ Ц(#„Ddh]„Da$gdД Ь$ Ц(#„D]„Da$gd4WЧ*ў6   > “  Ё М ь „ л ъ  K L  ™ Њ Е Ж Й Ц Ч rРТЧШцчtvСТ=єьфьмдмьєьєШєШМШМАЄ˜Є˜Є˜ЄŒЄ˜Є€qhє[єhД Ь6CJaJmHsHh5mH sH hhCJaJmH sH hh5mH sH hCJaJmHsHh*:CJaJmHsHh‡WЛCJaJmHsHhl‡CJaJmHsHhоyZCJaJmHsHhд"CJaJmHsHhоyZCJaJhmцCJaJhмбCJaJhД ЬCJaJhД ЬCJaJmHsH#=—ЋЬъPQZ[aƒ‰Š‹Œбпст$%єшмшдЩМАЄАЄАœŽœ‚œvœdvWvœLhњ!FhN3‹CJaJh‘xоhN3‹0JCJaJ#jh‘xоhN3‹CJUaJjhN3‹CJUaJhњ!FhN3‹6CJaJhN3‹hN3‹5CJH*aJhN3‹CJaJh3эCJaJmHsHhД ЬCJaJmHsHhД Ь5CJaJmHsHh’Vh’VCJaJhД ЬCJaJhUP/CJaJmHsHhЂ ~CJaJmHsHhœRaCJaJmHsH‹а%&'()*њњыщщщщ$„ўdh]„ўa$gdД ЬgdN3‹%&'()*ѕёэщэhJShњ!Fhтs'hN3‹hN3‹CJaJ,1hАа/ Ар=!А"А# $ %ААФАФ ФПDаЩъyљКЮŒ‚ЊKЉ lmaslov@agr.hrрЩъyљКЮŒ‚ЊKЉ ,mailto:lmaslov@agr.hrœ8@ёџ8 Д ЬNormal_HmH sH tHDA@ђџЁD Default Paragraph FontRi@ѓџГR  Table Normalі4ж l4жaі (k@єџС(No List6U@Ђё6 њ!F Hyperlink >*B*phџ* "џџџџƒ„ЊЋ)*+,56P Q Š ‹ а % & ' ( ) , 0€€0€€˜0€€˜0€€˜0€€˜0€€x˜0€€˜0€€€˜0€€˜0€€˜0€€˜0€€€˜0€€˜0€€ €˜@0€€x˜0€€˜0€€ˆ˜0€€˜0€€ˆ˜0€€˜0€€ €„Ћ+,56Q % & , Z@0ЅZ@0ЅZ@0ЅZ@0ЅX@0€X@0€Z@00€ @Ѕ4 Z@0€š€€k$6 =%* ‹* * с   * Xџ„џџђоjЬb•ѓоjьыєоj4ѕоjФ–#іоjдпоїоjє7шјоj\Эъъј!!, єўў((, B*€urn:schemas-microsoft-com:office:smarttags€country-region€8*€urn:schemas-microsoft-com:office:smarttags€City€9*€urn:schemas-microsoft-com:office:smarttags€place€=*€urn:schemas-microsoft-com:office:smarttags €PlaceType€=*€urn:schemas-microsoft-com:office:smarttags €PlaceName€ Iinpuv~„ŠŒŽ‘Љ ,56!&?HINPYБЖЫгктЗМНь38eo„лмъыLM™šЊЋЕЖЙКЦЧrsЂЃЌ­ТУЧШцhtv‘=>—˜ъ  A F Q Z \ a b ƒ … ‰  # , ‚„Љ,46“чvыP Q ‰ , P Q , , xШG!х д"тs'2,UP/*:њ!FJSцU’VоyZœRaˆdнJo+ v>:}Ђ ~l‡N3‹‘BšђЂЉ ЉП ЎthЖ‡WЛ4WЧД Ьмб}zвД-пMтmц3эџ@* @@џџUnknownџџџџџџџџџџџџG‡z €џTimes New Roman5€Symbol3& ‡z €џArial"qŠ№аhƒ]Ш&hШf}‚o-‚o-!№ ДДr4dю ю  3ƒ№H)№џ?фџџџџџџџџџџџџџџџџџџџџџП Ўџџ~Effect of Grapevine Solarization Technique on the Anthocyanin Content of Autochthonous Croatian Red Wine Teran (Vitis viniferaLunaLunaўџр…ŸђљOhЋ‘+'Гй0р˜ ,<HTd t€ œ Ј ДРШаиф€Effect of Grapevine Solarization Technique on the Anthocyanin Content of Autochthonous Croatian Red Wine Teran (Vitis viniferaLunaNormalLuna31Microsoft Word 10.0@.Yv@ђ6AэћШ@nфВЩќШ‚oўџеЭеœ.“—+,љЎDеЭеœ.“—+,љЎФ€ pxŒ”œЄЌ ДМФЬ д _ф Luna & cor-ю З Effect of Grapevine Solarization Technique on the Anthocyanin Content of Autochthonous Croatian Red Wine Teran (Vitis vinifera TitleЌ 8@ _PID_HLINKSфAd2mailto:lmaslov@agr.hr ўџџџўџџџ !"ўџџџ$%&'()*ўџџџ,-./012ўџџџ§џџџ5ўџџџўџџџўџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџRoot Entryџџџџџџџџ РFадЪЬЩќШ7€Data џџџџџџџџџџџџ1Tableџџџџ.WordDocumentџџџџ."SummaryInformation(џџџџџџџџџџџџ#DocumentSummaryInformation8џџџџџџџџ+CompObjџџџџџџџџџџџџjџџџџџџџџџџџџўџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџџўџ џџџџ РFMicrosoft Word Document MSWordDocWord.Document.8є9Вq