Naslov
Ionization mass spectrometry as a diagnostic tool

Autori
Potočnjak, Dalibor ; Barić Rafaj, Renata ; Lemo, Nikša ; Mrljak, Vladimir ; Žvorc, Zdravko ; Matijatko, Vesna ; Kiš, Ivana ; Harapin, Ivica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
13th ISACP, 10th ESVCP, 8th AECCP, 7th APP Congress Conference Program & Activities / Josep Pastor, Jose Ceron - Barcelona : ISACP and ESVCP, 2008, 62-62

Skup
13th ISACP, 10th ESVCP, 8th AECCP, 7th APP Congress

Mjesto i datum
Barcelona, Španjolska, 30.09.2008. - 03.10.2008

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
ionization mass spectrometry; pentaerythrityl tetranitrate (PETN)

Sažetak
Objective: A three-year-old dog was presented to the Clinic, because of its inability to work, weakness, depression and gastrointestinal symptoms. The dog had regular training for the tracking and detection of explosives. Mass spectrometry could provides valuable information in detection analites with very minute quantities, so we analysed the presence of explosive in the patients serum using this tehnique. Materials: After evaporation of the current phase of serum, the sample was analysed on an Ionscan mass spectrometer (Barringer Instruments, USA). The sample was introduced into the ionisation source (radioactive 63Ni), ions are extracted into the analyser region of the mass spectrometer where they are separated according to their mass (m) -to-charge (z) ratios (m/z) . The separated ions are detected and this signal is sent to a data system where the m/z ratios are stored together with their relative abundance for presentation in the format of a m/z spectrum . Result: The detector indicated a characteristic peak for explosive pentaerythrityl tetranitrate (PETN) . Conclusion: PETN is a nitroglycerin compound, one of the strongest known high explosives. By now, it is known that PETN is immediately metabolized after oral administration and is not detectable in the plasma after oral exposure. Interestingly enough, and in contrast to this, we found PETN in the serum of the dog after long-lasting exposure. Since the majority of mass spectrometric applications in the clinic are ; neonatal screening, haemoglobin analysis and drug testing, we could add to this the possibility of detection of extremely low quantities (10-15 moles) of unusual components from the blood.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA