Naslov
Histopathological evaluation of a dose-responsive tissue reaction associated with wow-formulated vaccine in chicken

Autori
Herak Perković, Vlasta, Tišljar, Marina, Tkalčić, Suzana, Ergotić, Neda

Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), stručni

Izvornik
European Society of Veterinary Pathology, 26th Annual Meeting, 17-21.09.2008., Programme and Book of abstracts, Hotel Palace Dubrovnik, Croatia / Grabarević, Željko, Hohšteter, Marko - Zagreb : European Society of Vetetrinary Pathology, Veterinary Faculty, University of Zagreb, 2008, 110-110

Skup
26th Annual Meeting European Society of Veterinary Pathology

Mjesto i datum
Dubrovnik, Hrvatska, 17.09.2008. - 21.09.2008

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
histopathology; chicken; wow-formulated vaccine; Newcastle Disease virus

Sažetak
This study investigates the tissue responses of a novel double-phase vaccinal emulsion formulated as water-in-oil-in-water (WOW). A total of 120 three-week-old White Leghorn Chickens, hatched from SPF eggs, were randomly assigned to four groups of 30 birds and monitored for the next 4 weeks. The chickens were vaccinated intramuscularly with 0.2, 0.5 and 1.0 ml of oil-based vaccine formulated as WOW emulsion (Pestikal, VETERINA, INC, Croatia) containing inactivated NDV. The control group was injected with 0.5 ml of sterile saline. Injection site tissue reaction was evaluated at 2 and 7-day intervals post inoculation. The severity of histopathological lesions was evaluated using a 1-4 scoring scale. Histopathological lesons in the pectoral muscles were correlated with the dose of injection. Predominant findings, regardless of the inoculated dose, were composed of macrophages, epitheloid cells and small interstitial cysts surrounded by the proliferating fibroblasts, lymphocytes and plasma cells. Lesions, characterized as oleogranuloma, were present in high dose WOW-inoculated chickens and persisted througout the 4 weeks of study. The manufacturer dose, based on the immunological response, is 0.5 ml. Our findings supported a dose of 0.5 ml of the WOW-formulated vaccinal emulsion as optimal in stimulating a local reaction needed for further humoral response.

Izvorni jezik
Engleski

Znanstvena područja
Javno zdravstvo i zdravstvena zaštita, Veterinarska medicina

POVEZANOST RADA