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\par }\pard \ql \li0\ri0\widctlpar\nooverflow\faroman\rin0\lin0\itap0 {
\par }\pard\plain \s5\qc \li0\ri0\sb240\sa60\widctlpar\nooverflow\faroman\outlinelevel4\rin0\lin0\itap0 \b\i\fs26\lang2057\langfe1033\cgrid\langnp2057\langfenp1033 {\b0\f41\fs24 Irena Kerekovi\'e6}{\b0\fs24\super 1*}{\b0\f41\fs24 , Stjepan Milardovi\'e6}{
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\par }{\i\f41\fs22\cf1 Department of General and Inorganic Chemistry, Faculty of Chemical Engineering and Technology, University of Zagreb, Maruli\'e6ev trg 19, HR-10000 Zagreb, Croatia}{\i\fs22\lang1050\langfe1033\langnp1050 
\par }{\i\fs22 *corresponding author:}{\i\fs22\cf1  irena.kerekovic@fkit.hr}{\fs22\lang1050\langfe1033\langnp1050 
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\par }\pard \qj \li0\ri0\widctlpar\nooverflow\faroman\rin0\lin0\itap0 {\fs22 Self-assembled monolayers (SAMs) are ordered mo
lecular assemblies formed by the adsorption of an active surfactant molecule on a solid surface [1]. SAMs are highly stable, well organized structures and can be used in corrosion prevention and development of various detection systems. Scientific interes
t
 in them begun in 1946. when Zisman reported adsorption of surfactant molecules on a metal surface [2], but only after 40 years SAMs showed their real potential with the discovery of the first gold-alkylthiolate monolayer by Nuzzo and Allara [3]. Electroc
hemical Impedance Spectroscopy (EIS) and cyclic voltammetry (CV) are suitable techniques for monitoring SAM properties. Methodology is based on comparison of the results obtained with the unmodified and modified electrode [4].
\par In this work we present results which can be employed for albumine sensor development. EIS measurements were done, for a bare gold electrode, in three-electrode electrochemical cell (W \endash  quartz crystal gold electrode, A \endash  Pt wire, R \endash 
 Ag/AgCl) at 0 V }{\i\fs22 vs.}{\fs22  Ag/AgCl. The frequency range was between 65 kHz and 1 Hz. Data points were collected at intervals of 5 points per dacade on a logarithmic scale, under excitation of sinusoidal wave with amplitude of \'b1
5 mV. The impedance spectra can be adequately fitted to an equivalent circuit involving
 solution resistance in series with a constant phase element. The buffer concentration was varied from 2 mM to 250 mM to determine which gives the best agreement with ideal behaviour (Helmholtz ideal capacitor model, where the phase angle at 1 Hz is 90}{
\fs22\super \'b0}{\fs22 ).
\par Self-assembled monolayer was prepared on a gold electrode, and the layer growth was investigated by impedance spectroscopy with same measurement parameters as for the bare electrode. The modification of the electrode was done in three steps (a) preparatio
n of cysteamine self-assembled monolayer, Au-CA, (b) activation of Au-CA by glutaraldehyde, Au-CA-GA, (c) functionalization of Au-CA-GA by nickel complex of nitrilotriacetic acid (Ni}{\fs22\super 2+}{\fs22 -NTA). (Fig. 1.)
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\par }{\fs22 
\par 
\par Fig. 1: Reaction steps in the preparation of three-layered SAM. 
\par 
\par 
\par }\pard \qj \li0\ri0\widctlpar\nooverflow\faroman\rin0\lin0\itap0 {\fs22 After preparation and characterization, the SAM was exposed to the solution of albumine and resulting impedance spectra indicated that albumine molecules got attached to the layer.

\par In the next step we plan to use QCM system (quartz crystal microbalance) for further validation of efficiency and performance of the developed sensor.
\par 
\par }\pard \ql \li0\ri0\widctlpar\faauto\rin0\lin0\itap0 {\fs20\lang1050\langfe1050\langnp1050\langfenp1050 
\par }\pard \qj \li0\ri0\widctlpar\nooverflow\faroman\rin0\lin0\itap0 {\fs22\lang1050\langfe1033\langnp1050 
\par {\listtext\pard\plain\fs22\lang1050\langfe1050\langnp1050\langfenp1050 \hich\af0\dbch\af0\loch\f0 [1]\tab}}\pard \qj \fi-426\li426\ri0\widctlpar\jclisttab\tx567\nooverflow\faroman\ls2\rin0\lin426\itap0 {\fs22\lang1050\langfe1050\langnp1050\langfenp1050 
A. Ulman, }{\i\fs22\lang1050\langfe1050\langnp1050\langfenp1050 Chem. Rev.}{\fs22\lang1050\langfe1050\langnp1050\langfenp1050  }{\b\fs22\lang1050\langfe1050\langnp1050\langfenp1050 96}{\fs22\lang1050\langfe1050\langnp1050\langfenp1050 , (1996), 1533.}{
\fs22 
\par {\listtext\pard\plain\fs22\lang1050\langfe1050\langnp1050\langfenp1050 \hich\af0\dbch\af0\loch\f0 [2]\tab}}{\fs22\lang1050\langfe1050\langnp1050\langfenp1050 W. C. Bigelow, D. L. Pickett, W. A. Zisman, }{
\i\fs22\lang1050\langfe1050\langnp1050\langfenp1050 J. Colloid Interface Sci.}{\fs22\lang1050\langfe1050\langnp1050\langfenp1050  }{\b\fs22\lang1050\langfe1050\langnp1050\langfenp1050 1}{\i\fs22\lang1050\langfe1050\langnp1050\langfenp1050 ,}{
\fs22\lang1050\langfe1050\langnp1050\langfenp1050 (1946)}{\i\fs22\lang1050\langfe1050\langnp1050\langfenp1050  }{\fs22\lang1050\langfe1050\langnp1050\langfenp1050 513.}{\fs22 
\par {\listtext\pard\plain\fs22\lang1050\langfe1050\langnp1050\langfenp1050 \hich\af0\dbch\af0\loch\f0 [3]\tab}}{\fs22\lang1050\langfe1050\langnp1050\langfenp1050 R .G. Nuzzo, D. L. Allara, }{\i\fs22\lang1050\langfe1050\langnp1050\langfenp1050 
J. Am. Chem. Soc.}{\fs22\lang1050\langfe1050\langnp1050\langfenp1050  }{\b\fs22\lang1050\langfe1050\langnp1050\langfenp1050 105}{\fs22\lang1050\langfe1050\langnp1050\langfenp1050 , (1983),}{\b\fs22\lang1050\langfe1050\langnp1050\langfenp1050  }{
\fs22\lang1050\langfe1050\langnp1050\langfenp1050 4481.}{\fs22 
\par {\listtext\pard\plain\fs22\lang1050\langfe1050\langnp1050\langfenp1050 \hich\af0\dbch\af0\loch\f0 [4]\tab}}\pard \qj \fi-567\li567\ri0\widctlpar\nooverflow\faroman\ls2\rin0\lin567\itap0 {\fs22\lang1050\langfe1050\langnp1050\langfenp1050 
S. Campuzano, M. Pedrero, C. Montemayor, E. Fat\'e1s, J. M. Pingarro\'f3n, }{\i\fs22\lang1050\langfe1050\langnp1050\langfenp1050 J. Electroanal. Chem}{\fs22\lang1050\langfe1050\langnp1050\langfenp1050 . }{
\b\fs22\lang1050\langfe1050\langnp1050\langfenp1050 586}{\fs22\lang1050\langfe1050\langnp1050\langfenp1050 , (2006), 112.}{\fs22 
\par }\pard \qj \li0\ri0\widctlpar\nooverflow\faroman\rin0\lin0\itap0 {\fs22\lang1050\langfe1050\langnp1050\langfenp1050 
\par 
\par 
\par 
\par }}