Naslov
FLUORESCENCE – PRINCIPLES AND APPLICATIONS

Autori
Galić, Nives

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
8th International Symposium and Summer School on Bioanalysis / - , 2008

Skup
8th International Symposium and Summer School on Bioanalysis

Mjesto i datum
Nitra, Slovačka, 01.06.2008. - 09.06.2008

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
fluorescence; mechanism; application

Sažetak
This lecture will give an introduction to the luminescence methods of molecular spectroscopy: fluorescence, phosphorescence, and chemiluminescence [1, 2]. The common principle of these techniques is the measurement of the emission spectra obtained when excited molecules decay to their ground states. Compared to absorption techniques, luminescence methods are extremely sensitive, with detection limits often one to three orders of magnitude smaller than those encountered in absorption spectroscopy. However, the number of substances which emitted light is limited. The brief overview of the fluorescence mechanism and quantitative fluorimetry (fluorescence efficiency or quantum yield, the inner filter effect, and quenching) will be given. The analytical methods for organic and inorganic analytes determination, such as direct, derivatisation and quenching methods will be discussed as well as the application of fluorescence in liquid chromatography and immunoassays.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA