Naslov
Ion-exchange chromatography separation of the detergent and the solvent from immunoglobulins after solvent-detergent treatment

Autori
Gebauer, Branka ; Šimić, Mirjana ; Branović, Karmen ; Treščec, Anđa ; Benko, Bojan

Izvornik
Journal of chromatography A (0021-9673) 852 (1999), 1; 83-86

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
solvent-detergent method; immunoglobulins; triton x-100; tributyl phosphate

Sažetak
For inactivation of lipid-enveloped viruses during the immunoglobulin production, the solvent-detergent (S/D) method was applied. Tri-n-butyl phosphate (solvent) and Triton X-100 (detergent) were removed from S/D treated immunoglobulins by ion-exchange chromatography on Q-Sepharose Fast Flow (FF). During the chromatographic procedure immunoglobulins remained bound on a Q-Sepharose EF, whereas solvent and detergent were eluted by washing with starting buffer. Elution of immunoglobulins was achieved by increasing the ionic strength of the starting buffer. The final immunoglobulin preparation contained less than 10 mu g/ml of Triton X-100 and less than 2 mu g/ml tri-n-butyl phosphate. It was confirmed that the S/D procedure did not cause a significant change in polymers and specific antibodies content. Immunoglobulin classes were also not affected by the same procedure. (C) 1999 Elsevier Science B.V. All rights reserved. [References: 14]

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Farmacija

POVEZANOST RADA