Naslov
Spatial and seasonal variability of proteolytic genes and activity in an arable field

Autori
Mrkonjić Fuka, Mirna ; Engel, Marion ; Munch, Jean Charles ; Schloter, Michael

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Munich Environmental Microbiology Meeting / - München, 2005, 13-13

Skup
Environmental Microbiology Meeting

Mjesto i datum
München, Njemačka, 29.11.2005

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
soil proteolytic activity; sub; npr; 16S rRNA; T-RFLP; Real-Time PCR

Sažetak
Proteolytic activity has been extensively investigated in the last few decades but almost nothing is known about size, diversity, distribution and regulatory mechanisms of the gene pool involved in soil proteolytic processes. The indigenous bacterial communities harbouring genes encoding for extracellular proteases play a critical role in regulating proteolysis and nitrogen mobilization in soils. Thus there is a major interest in exploring factors influencing its size, diversity and activity. For this purpose an arable field (Research Farm Scheyern, Oberbayern) under uniformly integrated management and cropped with potatoes was studied. The field shows pronounced soil heterogeneity with four different soil types. In April, July and October 2003 soil samples were taken from the four soil types at three different depths: D1 (≈ 0 - 10 cm), D2 (≈ 10 - 30 cm) and D3 (≈ 30 - 40 cm) according to the horizontation of the soil. We applied culture independent methods for quantification and diversity analysis of subtilisin gene (sub) and neutral metalloprotease gene (npr) both enconding for extracellular proteases as well as 16S rRNA gene representing the size of bacterial population.

Izvorni jezik
Engleski

Znanstvena područja
Poljoprivreda (agronomija)

POVEZANOST RADA