Naslov
Quantification of functional genes from procaryotes in soil by PCR

Autori
Sharma, Shilpi ; Radl, Viviane ; Hai, Brigitte ; Kloos, Karin ; Mrkonjić Fuka, Mirna ; Engel, Marion ; Schauss, Kristina ; Schloter ; Michael

Izvornik
Journal of microbiological methods (0167-7012) 68 (2007); 445-452

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, pregledni rad, znanstveni

Ključne riječi
Quantitative PCR; Real-time PCR; MPN-PCR; Competitive PCR; Nucleic acid extraction; Microbial activity

Sažetak
Controlling turnover processes and fluxes in soils and other environments requires information about the gene pool and possibilities for its in situ induction. Therefore in the recent years there has been a growing interest in genes and transcripts coding for metabolic enzymes. Besides questions addressing redundancy and diversity, more and more attention is given on the abundance of specific DNA and mRNA in the different habitats. This review will describe several PCR techniques that are suitable for quantification of functional genes and transcripts such as MPN-PCR, competitive PCR and real-time PCR. The advantages and disadvantages of the mentioned methods are discussed. In addition, the problems of quantitative extraction of nucleic acid and substances that inhibit polymerase are described. Finally, some examples from recent papers are given to demonstrate the applicability and usefulness of the different approaches.

Izvorni jezik
Engleski

Znanstvena područja
Poljoprivreda (agronomija)

POVEZANOST RADA