Naslov
DNA transfer from genetically modified plants to a soil bacterium

Autori
Ćurković Perica, Mirna ; Šeruga, Martina ; Garcia-Gonzales, R. ; Mansouri, H. ; Message, B. ; Leach, F. ; Tepfer, D.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
2. hrvatiski biološki kongres

Mjesto i datum
Zagreb, Hrvatska, 27.09.2003. - 02.10.2003

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Nije recenziran

Ključne riječi
DNA transfer; genetically modified plants; nptII

Sažetak
Species are usually considered to be sexually isolated, but horizontal gene transfer is easily demonstrated in bacteria and likely occurs in higher organisms. Approximately 40 species of soil bacteria are known to acquire DNA from their environment, yet attempts to directly observe transfer of DNA from plants to bacteria have often produced negative or equivocal results. In our experiments, DNA transfer was demonstrated from six species of donor plants to the soil bacterium, Actinobacter spp. BD413, using neomycin phosphotransferase (nptII) gene as a marker for homologous recombination. Neomycin phosphotransferase is an enzyme that gives resistance to antibiotics neomycin and kanamycin. In tobacco carrying a plastid insertion of nptII, transfer was detected with 0, 1g of disrupted leaves and in oilseed rape carrying a nuclear insertion with a similar quantity of roots. Transfer from disrupted leaves occurred in sterile soil and water, without the addition of nutrients. It was dose-dependent and sensitive to DNase, and mutations in the plant nptII were recovered in receptor bacteria. DNA transfer using intact oilseed and morning glory roots, and tobacco and Arabidopsis plants in vitro was also detected, but with greater variability. Transfer varied with plant genome size and the number of repeats of the marker DNA in the donor plant. These results imply the possibility that DNA transfer from genetically modified plants to bacteria could occur in nature.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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