Naslov
Distribution of major gangliosides in adult mouse brain

Autori
Heffer-Lauc, Marija ; Viljetić, Barbara ; Schnaar, Ronald L. and Lauc, Gordan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of abstracts - Glycoproteomics: protein modifications for versatile functions / Dumić, Jerka ; Flogel, Mirna - Zagreb, 2005, 75-75

ISBN
953-6256-54-1

Skup
Satellite meeting to the 30th FEBS Congress and 9th IUBMB Conference - Glycoproteomics: protein modifications for versatile functions

Mjesto i datum
Dubrovnik, Hrvatska, 28.06.2005. - 30.06.2005

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
complex gangliosides; mouse; brain; distribution

Sažetak
Since the early days of ganglioside work numerous biochemical and immunohistochemical studies have addressed the question of exact location of gangliosides. Since gangliosides are highly conserved and thus generally not immunogenic, the lack of specific antibodies has burdened the field until recently when highly specific antibodies were raised in mice deficient for complex gangliosides. Fixation artifacts and the pheenomenon of ganglioside jumping between different cells and tissues have further complicated the picture. After developing a procedure that minimized effects of all these factors, we have analyzed distribution of GM1, GD1a, GD1b, and GT1b in the mouse brain. GM1 appears to be localized primarily in myelinized brain regions, i.e. oligodendroglia. The other three gangliosides were found to have very similar distributions: They were present on virtually all neural and glial cells in the brain, but not on fibers or dendroglia. GD1a was present only on neurons, while GD1b and GT1b were mostly present on glial cells that delineate specific regions of the mouse brain. This was particularly visible in pons and medulla (that consist nearly exclusively of fibers), and where GD1b and GT1b were found on boundaries between individual fibers.

Izvorni jezik
Engleski

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