Pregled bibliografske jedinice broj: 350831
Trigonopsis variabilis D-Amino Acid Oxidase:Control of Protein Quality and Opportunities for Biocatalysis through Production in Escherichia coli
Trigonopsis variabilis D-Amino Acid Oxidase:Control of Protein Quality and Opportunities for Biocatalysis through Production in Escherichia coli // Applied and Environmental Microbiology, 73 (2007), 1; 331-332 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 350831 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Trigonopsis variabilis D-Amino Acid Oxidase:Control of Protein Quality and Opportunities for Biocatalysis through Production in Escherichia coli
Autori
Dib, Iskandar ; Stanzer, Damir ; Nidetzky, Bernd
Izvornik
Applied and Environmental Microbiology (0099-2240) 73
(2007), 1;
331-332
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
D-amino acid oxidase; biocatalysis; microheterogeneity; recombinant production; oriented imobilization
Sažetak
Trigonopsis variabilis d-amino acid oxidase accounts for 35% of Escherichia coli protein when added d-methionine suppresses the toxic activity of the recombinant product. Permeabilized E. coli cells are reusable and stabilized enzyme preparations. The purified oxidase lacks the microheterogeneity of the natural enzyme. Oriented immobilization of a chimeric oxidase maintains 80% of the original activity in microparticle-bound enzymes.
Izvorni jezik
Engleski
Znanstvena područja
Biotehnologija
POVEZANOST RADA
Projekti:
058-0580477-0374 - Proizvodnja biološki aktivnih tvari pomoću selekcioniranih kvasaca (Stanzer, Damir, MZOS ) ( CroRIS)
Ustanove:
Prehrambeno-biotehnološki fakultet, Zagreb
Profili:
Damir Stanzer
(autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE
