Naslov
SDS-PAGE separation of hepatopoietin protein bands

Autori
Topić, Elitabeta ; Petrač, Dubravko ; Paripović, Roberta ; Gluhak, Jelica ; Zadro, Renata ; Slijepčević, Milivoj ; Ruhenstroth-Bauer, Gerhard.

Izvornik
Clinica e Laboratorio (0391-2035) 19 (1995), 2; 41-46

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
hepatopoietin; hepatocyte gorwth factor; SDS-PAGE

Sažetak
Human plasma hepatopoietin (hPHP) extract was isolated for 72 liver disease patients and 25 healthy volunteers. Protein bands of hepatopietin extract were electrophoretically separated by SDS-PAGE modified so as to allow good separation of protein between Mr 30000 and 50000. The electrophoretic pattern showed three protein bands in this range, i.e. A, B, and C, with Mr of about 41700, 38000 and 33800, respectively. Positive band A was found in 92%, band B in 97%, and band C in 85% of patients. In the control group, 80% of subjects had positive band A, 88% band B, and 80% band C. When the bands were compared to the results of biochemical parameters commonly use to test the liver function, they showed the highest frequency of band A in patients with ALT and LDH values by 3 SD anove the mean, whereas the highest frequency of band B was found in patients with TB, AP, AST, gamma GT, AFP and CHE values by 3 SD exceding the mean value of the reference interval. Band C was observed to have lowest frequency, regrdless of the biochemical parameter evaluated.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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