Naslov
4-hydroxynonenal enhances permeability of in vitro intestinal barrier model

Autori
Borović Šunjić, Suzana ; Štroser, Marina ; Živković, Emilija ; Žarković, Neven

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the COST Meeting "Oxygen, stress and lipids" / - , 2007

Skup
COST Meeting "Oxygen, stress and lipids"

Mjesto i datum
Dubrovnik, Hrvatska, 26.10.2007. - 27.10.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
CaCo-2; differentiation; 4-hydroxynonenal; HNE; intestinal barrier; antioxidants; oxidative stress; gastrointestinal tract

Sažetak
Human colon adenocarcinoma cell line CaCo-2 was shown to undergo spontaneous in vitro enterocytic differentiation leading to the formation of cell monolayer with several morphological an functional characteristics of mature enterocytes. Gastrointestinal tract is often exposed to harmful compounds originating from diet ; they can cause oxidative stress and intestinal dysfunction. Such dysfunction can make the intestinal barrier permeability greater for toxins, bacteries, harmful products of digestion, free radicals and second messangers of free radicals like 4-hydroxynonenal (HNE). HNE is one of major lipid peroxidation product, and because of its lipophilic caracter, it can be readily absorbed in the intestine or can accumulate in membranes causing phospholipid and protein damage. Cell monolayer was good developed after 15th day and represents intestinal barrier model. HNE impaired function of intestinal barrier model and caused increase in its permeability. With this study, we have shown that in vitro model of intestinal barrier could be used for oxidative stress research.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA