Naslov
Differential expression of the perforin in human peripheral blood lymphocytes of patients with lung carcinoma

Autori
Marija Petković, Ines Mrakovčić-Šutić, Ingrid Belac-Lovasić, Franjo Lovasić

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
5th Central European Oncology Congress / - , 2007

Skup
5th Central European Oncology Congress, CEOC 2007

Mjesto i datum
Opatija, Hrvatska, 20.06.2007. - 23.06.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
lung carcinoma; cellular immunity; perforin; NKT cells

Sažetak
Current data shown that the development of lung carcinoma is a multistep process that involves a successive loss of genes, environmental factors and behavioral habits. The aim of this study was to examine alteration of cell mediate immunity and cytolytic molecule perforin in different lymphocyte subpopulation in peripheral blood of lung cancer patients. Venous blood samples were taken and peripheral blood leukocytes (PBL) were isolated. Phenotype of lymphocytes and perforin expression were analyzed using intracellular and surface immunofluoresecncy and flow cytometric analysis (FACSCalibur). Statistical analysis was done in computer program Statistica 7.1 (StatSoft, Inc., Tulsa, OK, USA). Graphs and tables were done in computer program Microsoft Excel. The results demonstrated that 10-15% of PBL in all examine group are perforin positive (total perforin is significantly lower than in control group). Surprisingly, percentages of double positive cells showed significant decrease in all examine lymphocyte subpopulations (CD3+ P+, CD4+P+, CD8+P+, CD16+P+ and CD56+P+ cells) compared to controls. These results suggest that human perforin is differentially expressed in distinct sub-populations of peripheral blood lymphocytes of patients with lung carcinoma, implicating the important role of perforin positive cells in na&iuml ; ve immune response and cytotoxic activity of peripheral blood lymphocytes.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

POVEZANOST RADA