Naslov
AFM as a tool for revealing cell morphology at micro- and nanoscale

Autori
Mišić, Tea ; Svetličić, Vesna ; Viljetić, Barbara ; Kalanj Bognar, Svjetlana ; Heffer Lauc, Marija

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts of the Second Croatian Congress of Neuroscience ; u: Neurologia Croatica. Supplement 56 (2007) (S2) / Ivkić, Goran ; Judaš, Miloš ; Klarica, Marijan ; Kostović, Ivica ; Šimić, Goran ; Petanjek, Zdravko - Zagreb : Denona, 2007, 138-138

Skup
Croatian Congress of Neuroscience (2 ; 2007)

Mjesto i datum
Zagreb, Hrvatska, 18.05.2007. - 19.05.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
AFM ; cell morphology

Sažetak
Atomic force microscopy (AFM) belongs to the broad family of scanning probe microscopic techniques (SPMs) in which a proximal probe is exploited to investigate the properties of surfaces. AFM provides real topographic images of the sample surfaces in three dimensions with a subangstroem vertical resolution, and with lateral resolution superior to 1 nm. Besides providing topographic data, AFM can also be used for measuring nanomechanical properties of the samples as well as intra- and intermolecular forces. In biological applications, the most appealing advantage of the AFM as a high resolution microscope is that it allows measurements of native biological samples in physiological like condtions, thus obviating complex sample preparation procedures typical for SEM and TEM techniques. In this study, we used AFM in the analysis of human neuroblastoma cell line SH-SY5Y cellular morphology. Neuroblastoma cells were grown on silanized glass coverslips, and cultivated in MEM medium supplemented with 10% fetal bovine serum. For AFM analysis, cells were briefly washed with phosphate buffer saline (PBS) and fixed with 2% paraformaldehyde in PBS. AFM images were collected using Multimode AFM with Nanoscope IIIa controller (Veeco Instruments, Santa Barbara, CA) with a vertical engagement (JV) 125 um scanner. Contact imaging mode was performed in air using minimum force to maintain contact between the probe and the scanned surface. We present AFM images of human neuroblastoma cell line SH-SY5Y. Neuronal-like morphology of SH-SY5Y cells, characterized by cell bodies with neuritic extensions, is viewed in fascinating detail. Cell surface was characterized down to molecular scale (lipid aggregates-membrane rafts). Cell dimensions were accurately measured including cell height, which is useful for determining cell volume. Membrane pores were captured showing excreting activity. Membrane pores were in the size range of 200-500 nm. Studies of different neuronal-like populations fing AFM to be a powerful tool for revealing cellular morphology at micro- and nanoscale.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Temeljne medicinske znanosti

Napomena
Časopis je indeksiran u Neuroscience Citation indeksu i EMBASE/Excerpta Medica.

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