Naslov
THE INVOLVEMENT OF PERFORIN EXPRESSION IN PERIPHERAL BLOOD OF BURNED PATIENTS

Autori
Aleksandra Pirjavec-Mahić, Ines Mrakovčić-Šutić, Miljenko Uravić

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
12th Congress of the European Burns Association (EBA) / - , 2007

Skup
12th Congress of the European Burns Association (EBA)

Mjesto i datum
Budimpešta, Mađarska, 12.07.2007. - 15.07.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
burns; perforin; innate immunity; peripheral blood lymphocytes

Sažetak
Introduction: Cytotoxic T cells (CTL) and natural killer (NK) cells induce cell death of their target cells either by the release of perforin and granzymes or by the surface interaction between Fas ligand and Fas. Both these lytic pathways induce apoptosis of neoplastic and an altered-self cell. Methods: Venous blood samples were taken and peripheral blood leukocytes were isolated. Phenotype of lymphocytes and perforin expression were analyzed using intracellular and surface immunofluoresecncy and flow cytometry. Results: Our data demonstrated that 10-15% of PBL in patients are perforin positive. Percentage of all double positive cells showed significant decrease in patients with burns injuries in comparation with healthy volunteers, showing the possible prognostic recovery value of these findings. Conclusions: Our results suggest the important role of perforin positive cells in na&iuml ; ve immune response and cytotoxic activity of peripheral blood cells of patients with burn injuries.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

POVEZANOST RADA