Naslov
The role of chronic exposure to gasoline and diesel on cell mediated immunity of people situated near gasoline industry

Autori
Bulog, Aleksandar ; Mrakovčić-Šutić, Ines ; Mićović, Vladimir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
13th International Congress of Immunology ICI : Collection of papers from Thirteenth International Congress of Immunology / Kalil, Jorge ; Cunha-Neto, Edecio ; Vicente-Rizzo, Luiz - Bolonja : Medimond International Proceedings, 2007

ISBN
978-88-7587-379-0

Skup
13th International Congress of Immunology - ICI

Mjesto i datum
Rio de Janeiro, Brazil, 21.08.2007. - 25.08.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
air pollutants; environmental diseases; innate immunity; perforin

Sažetak
Urban air pollutants, particularly benzene, toluene, ethyl benzene, isomeric xylenes (BTEX) exhaust particles are known to contribute to the increased prevalence of many allergic and environmental diseases and multiple chemical sensitivity or other related syndromes, as a result of an abnormal immune response based on environmental damage of lymphocyte subsets. The aim of this study was to examine alteration of cell mediate immunity and cytolytic molecule perforin in different lymphocyte subpopulation in peripheral blood of subjects situated near gasoline industry compared with healthy laboratory donors. Venous blood samples were taken from all examinees and peripheral blood leukocytes (PBL) were isolated. Phenotype of lymphocytes and perforin expression were analyzed using intracellular and surface immunofluoresecncy and flow cytometry (FACSCalibur). Statistical analysis was done in computer program Statistica 7.1 (StatSoft, Inc., Tulsa, OK, USA). Graphs and tables were done in computer program Microsoft Excel. The results demonstrated that 17% of PBL in all examine group are perforin positive (total perforin is significantly lower in exposure than in control group). Percentages of double positive cells showed significant decrease in all examine lymphocyte subpopulations (CD3+ P+, CD4+P+, CD8+P+, CD16+P+ and CD56+P+ cells) compared to controls. Our data suggest that human perforin is differentially expressed in distinct sub-populations of peripheral blood lymphocytes of people exposure to gasoline and diesel, implicating the important role of perforin positive cells in naï ; ve immune response and cytotoxic activity of peripheral blood lymphocytes.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti, Javno zdravstvo i zdravstvena zaštita

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