Naslov
Deficient expression of trefoil peptides in knock-out mice causes hearing loss

Autori
Lubka, M, Baus Lončar, Mirela ; Müller, M ; Löwenheim, H ; Knipper, M ; Pfister, M ; Blin N.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
GfH ÖGH Tagung 2007, Book of abstracts / - , 2007

Skup
18.jahrestagung dr Deutchen Gesellschaft für Humangenetik (GfH)

Mjesto i datum
Bonn, Njemačka, 07.03.2007. - 10.03.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
TFF3 knock-out; hearing loss; inner ear

Sažetak
Introduction: In mammals trefoil factor family (TFF) consists of three members: TFF1, TFF2 and TFF3. They are major secretory products of mucous epithelia and are usually expressed in association with mucins. They play a multifunctional role in cytoprotection, apoptosis, and immune response. They are predominantly expressed in the gastrointestinal tract but also in other tissues like respiratory tract, salivary glands, uterus, conjuctiva and brain. We show for the first time the expression of TFF2 and TFF3 proteins in the mouse inner ear and present data from hearing measurements of Tff3 and Tff2 knock_out animals. Methods: We used quantitative real time PCR (qPCR) with primers specific for mouse Tff2 and Tff3 genes to analyze their expression in the in_ ner ear. To show the localization of both proteins immunohistochemistry using antibody specific for mouse TFF2 and TFF3 proteins was performed. Auditory evoked brainstem responses (ABR) were measured in wild_type and Tff3 ko animals aged 3 weeks up to 1 year and on 3 months old Tff2 ko animals to assess possible functional changes. Results: Data obtained from qPCR pointed to a specific expression pattern. Immunohistochemical experiments demonstrated TFF2 in the spiral ligament and TFF3 in the stria vascularis of the inner ear. ABR measurements revealed that young Tff3 ko mice showed a flat hearing loss of about 15 dB below 10 kHz when compared to age matched wild_type animals. At one year of age ko mice showed a flat hearing loss of about 30 dB below 10 kHz and an increasing loss towards higher frequencies (50 dB at 32 kHz). At this age wild_type animals had a high_frequency loss of about 25 dB. Tff2 ko mice showed normal hearing at 3 month of age. Conclusions: Beside secreting epithelia of various organs, trefoil factor 2 and 3 are also expressed in the inner ear. Tff3 ko mice show an accelerated presbyacusis and a flat hearing loss below 10 kHz. The situation in Tff2 ko mice still needs to be evaluated.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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