Naslov
The effect of selenium pre-treatment on selenium content and lipid peroxidation in cadmium exposed suckling rats

Autori
Lazarus, Maja ; Orct, Tatjana ; Jurasović, Jasna ; Blanuša, Maja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstacts, 4th International Conference on Metals and Genetics ICMG 2008 / - Pariz, 2008

Skup
4th International Conference on Metals and Genetics ICMG 2008

Mjesto i datum
Pariz, Francuska, 21.07.2008. - 24.07.2008

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
cadmium exposure; lipid peroxidation; selenium pre-treatment; suckling rats

Sažetak
Higher and prolonged gastrointestinal absorption of cadmium (Cd) in newborns compared to adults, and its nature to accumulate in the critical tissues gives importance to early postnatal studies specially in conditions of increased environmental exposure to Cd. Developing brain has been recognized as particularly vulnerable to toxic effects of Cd. Cadmium can be transported through immature blood-brain barrier (BBB) and cause oxidative destruction of membrane polyunsaturated fatty acids (i.e. lipid peroxidation, LPO) in brain and the other tissues as well. Selenium (Se) has known antioxidative potential being part of free radical scavenging enzyme glutathione peroxidase (GSH-Px). In this study we investigated levels of LPO and Se in Cd exposed suckling pups orally pre-treated with Se. Our earlier results evidenced the lower tissue Cd levels in Se supplemented suckling pups. Six days old suckling Wistar rats were pre-treated with Se (as Na2SeO3) and after four days, rats were orally and concurrently treated for another five days with Cd (as CdCl2xH2O) and Se in equimolar amounts (8 μ mol/kg b.w./day, each). Lipid peroxidation in kidney, liver and brain homogenates was quantified using the thiobarbituric acid reactive substances (TBARS) assay. Results confirmed significant decrease in tissue Cd levels in Se supplemented suckling rats (Cd+Se-treated group) in comparison to Cd exposed rats (Cd only). The analysis of Se content in different pups´ tissues indicated significant effect of oral treatment with Se (Se only) on the Se concentration in blood, kidney, liver and brain. In the presence of concurrent Cd exposure (Cd+Se group) Se concentration in blood, brain and kidney was significantly lower when compared to Se-treated animals (Se only). There was no significant increase in LPO in Cd treated group compared to control. However, pretreatment with Se (Se+Cd group) caused statistically significant change in LPO level in liver and brain in comparison to Cd exposed group. These results indicate some Cd/Se interaction at the investigated sites.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Javno zdravstvo i zdravstvena zaštita

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