Naslov
Expression of endothelial selectin ligands on human leukocytes following dive

Autori
Glavaš, Duška ; Markotić, Anita ; Valić, Zoran ; Kovačić, Nataša ; Palada, Ivan ; Martinić, Roko ; Brešković, Toni ; Baković, Darija ; Brubakk, Alf O. ; Dujić, Željko

Izvornik
Experimental Biology and Medicine (1535-3702) 233 (2008), 9; 1181-1188

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
scuba diving; endothelial function; monocyte; sialyl-Lewis x; ultrasound

Sažetak
The fact that impaired endothelial-dependent vasodilatation after scuba diving often occurs without visible changes in the endothelial layer implies its biochemical origin. Since Lewis x (CD15) and sialyl-Lewis x (CD15s) are granulocyte and monocyte carbohydrate antigens recognized as ligands by endothelial selectins, we assumed that they could be sensitive markers for impaired vasodilatation following diving. Using flow-cytometry, we determined CD15+ and CD15s+ peripheral blood mononuclear cells of eight divers, 30 min before and 50 min after a single dive to 54 m for 20 min bottom time. The number of gas bubbles in the right heart was monitored by ultrasound. Gas bubbles were seen in all 8 divers, with the average number of bubbles/cm2 1.9± 1.9. Proportion of CD15+ monocytes increased two fold after dive as well as subpopulation of monocytes highly expressing CD15s, the absolute number of monocytes was slightly but no significantly increased after dive, whereas absolute number of granulocytes was markedly elevated (up to 61%) after dive. There was not correlation between CD15+ monocytes expression and bubbles production (p = , 555 ; r = -, 2471) as well as between monocytes highly expressing CD15s and bubbles production (p = , 288 ; r = , 42983. This suggests that biochemical changes induced by scuba diving primary activate existing monocytes rather than increase the number of monocytes at a time of acute arterial endothelial dysfunction.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

POVEZANOST RADA