Naslov
Perforin expression in peripheral blood lymphatic cells of patients subjected to laparoscopic or open cholecystectomy

Autori
Grbas, Harry ; Mrakovčić-Šutić, Ines ; Depolo, Arsen ; Radošević-Stašić, Biserka

Izvornik
Mediators of inflammation (0962-9351) 2009 (2009); 1-11

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
open and laparoscopic cholecystectomy; perforin; cytotoxic lymphocytes; cholestasis
(klasična i laparoskopska kolecistectomija; perforin; citotoksični limfociti; kolestaza.)

Sažetak
Perforin (P)-related characteristics of cytotoxic T lymphocytes and natural killer cells were investigated in peripheral blood of patients subjected to open (OC ; n=23) or laparoscopic cholecystectomy (LC ; n=21) and healthy controls (n=20). Blood samples were obtained preoperatively and 24 h after the surgeries and the data were correlated with the intensity of cholestasis and concomitant inflammation, determined by functional hepatic tests. Postoperative differences were found to be minimal: OC decreased only the percentage of CD56+ cells, while LC decreased the fraction of CD8+P+ cells and augmented the mean fluorescence intensity of P in CD56 cells. Patients elected for OC had, however, higher preoperative numbers of total P+, CD3+P+ and CD4+P+ cells than patients elected for LC and healthy controls, while both groups of patients, preoperatively, had lower fraction of CD16+P+ and CD56+P+ cells. These changes were in high correlation with blood concentrations of CRP, AP and ALT, emphasizing the link between the preoperative cholestasis and inflammation and P-dependent cytotoxic mechanisms.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

Napomena
Article ID 125152.

POVEZANOST RADA