Naslov
The determination of sulfonamides in honey by high performance liquid chromatography-mass spectrometry method (LC/MS)

Autori
Krivohlavek, Adela ; Šmit, Zdenko ; Baštinac, Martina ; Žuntar, Irena ; Plavšić, Franjo

Izvornik
Journal of separation science (1615-9306) 28 (2005), 13; 1434-1439

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
honey; sulfonamides; SPE; LC/MS

Sažetak
The sulfonamides (SAs) are stable chemotherapeutics used against the bacterial disease affecting bees, known as American foulbrood (Bacillus larvae), so their residues could appear in the honey of treated bees. Their presence at a concentration above the limit value could be a potential danger to human health. Therefore, a simple, rapid, and reliable method for determination of 11 available SAs in honey was optimized. The samples were homogenized and cleaned with extraction on solid phase by means of Chromabond C18 end-capped cartridge followed by LC/MS analyses. A detection limit of 25 ug/kg was achieved for all analytes. The repeatability of the method was proven and the optimal parameters for temperature and pH of the mobile phase and acetic buffer, respectively, were determined. In this study, 20 samples of domestic honey were included. Six of the analyzed samples were positive, but all results were below the Croatian permissible limit value (100 ug/kg).

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Javno zdravstvo i zdravstvena zaštita, Farmacija

Napomena
Rad je kao poster prezentiran na skupovima 10th International Symposium of Separation Science, održanom 2004.g., Opatija, Hrvatska i Apimondia Ireland, održanom od 21.-26.08.2005.g., Dublin, Irska ; objavljen u Knjigama sažetaka.

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