Naslov
Citrus tristeza virus indexing and elimination from Satsuma mandarin (Citrus unshiu Marc.) cv. Kuno

Autori
Hartl Musinov, Danijela ; Hančević, Katarina ; Rošin, Jakša ; Gatin, Živko

Izvornik
Pomologia Croatica (1330-6626) 12 (2006), 4; 285-294

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
bioindicator; micrografting; certification

Sažetak
The main grown citrus crop in Croatia is Satsuma mandarin (Citrus unshiu Marc.). The preliminary surveys have shown widespread occurrence of the most devastating citrus pathogen, Citrus tristeza virus (CTV) in Croatia. In this research four Satsuma mandarin cultivars: Chahara, Ichimaru, Kuno and Zorica Rana were biologically tested for the presence of CTV on Mexican lime (Citrus aurantifolia Swingle) bioindicator. Displayed symptoms strongly suggest the existence of mild CTV strains in four tested mandarin cultivars. Laboratory diagnostic methods (DTBIA, DAS-ELISA, IC/RT-PCR) confirmed these findings. Plants of cv. Kuno mandarin underwent sanitation treatments which consisted of thermotherapy and shoot-tip grafting (STG ; micrografting) on previously in vitro germinated Troyer citrange [Poncirus trifoliata (L.) Raf. x Citrus sinensis (L.) Osbeck)] rootstock. Tristeza-virus was eliminated from 42% of infected ’ Kuno’ mandarins (confirmed by DTBIA). Completion of the above mentioned procedures will help in development and implementation of citrus certification program in Croatia which would ensure the production of high-quality citrus propagating material in both sanitary and pomological aspects.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Biotehnologija

POVEZANOST RADA