Naslov
Dose- and Mode-Dependent Effect of Halogen Dental Curing Blue Light on the V79-Cell Line

Autori
Jukić Krmek, Silvana ; Trošić, Ivančica ; Pavičić, Ivan ; Keros, Jadranka ; Simeon, Paris ; Malčić, Ana ; Prpić-Mehičić, Goranka

Izvornik
Collegium antropologicum (0350-6134) 32 (2008), 3; 913-918

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Halogen lamp ; photopolymerization ; cell culture ; viability ; colony forming ability ; proliferation

Sažetak
The aim of study was to evaluate the time and dose dependence effect of halogen light curing unit (Elipar TriLight, ESPE Dental AG, Germany) at the disposed condition modes in vitro. The influence of medium mode (M), exponential (E) and standard (S) illumination during 20, 40 and 80 sec on the cell viability, colony forming ability and proliferation of V79 cell culture was investigated. Trypan blue exclusion test was used to determine cell viability, both, in the treated and control cell samples. Colony forming ability was assessed for each exposure time and mode by colony count on post-exposure day 7. Cell proliferation was determined by cell counts for each time and mode of exposure during five post-exposure days. The results were analyzed using StatSoft 2003 package. Viability of cells was not affected by blue light in view of exposure time and modes. Regardless to exposure or illumination modes, colony forming ability were slightly but not significantly depressed. Cell proliferation was decreased in samples exposed to M mode for 80 sec on post-exposure day 3 and 4 (p<0.05). On the same post-exposure days the proliferation of cells exposed to E and S mode showed a significant inhibition after 20, 40 and 80 sec of exposure (p<0.05). Disrupted cellular functionality and no significant decrease in colony forming ability of V79 cells in addition to time- and dose dependent significant inhibition of cell proliferation might be ascribed to the photocuring blue light activity and/or changes in temperature during the course of experiment in vitro.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Dentalna medicina

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