Naslov
Dual targeting of organellar seryl-tRNA synthetase to maize mitochondria and chloroplasts

Autori
Rokov-Plavec, Jasmina ; Dulić, Morana ; Duchene, Anne-Marie ; Weygand-Durasevic, Ivana

Izvornik
Plant Cell Reports (0721-7714) 27 (2008), 7; 1157-1168

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
seryl-tRNA synthetase; maize; dual targeting; mitochondria; chloroplast; stromule

Sažetak
Aminoacyl-tRNA synthetases (AARSs) play a critical role in translation and are, thus, required in three plant protein-synthesizing compartments: cytosol, mitochondria and plastids. A systematic study had previosly shown extensive sharing of organellar AARSs from Arabidopsis thaliana, mostly between mitochondria and chloroplasts. However, distribution of AARSs from monocot species, such as maize, has never been experimentally investigated. Here we demonstrate dual targeting of maize seryl-tRNA synthetase (SerZMo) into both mitochondria and chloroplasts using combination of complementary methods, including in vitro import assay, transient expression analysis of green fluorescent protein (GFP) fusions and immunodetection. We also show that SerZMo dual localization is established by the virtue of an ambiguous targeting peptide. Full-length SerZMo protein fused to GFP is targeted to chloroplast stromules, indicating that SerZMo protein performs its function in plastid stroma. The deletion mutant lacking N-terminal region of the ambiguous SerZMo targeting peptide was neither targeted into mitochondria nor chloroplasts, indicating importance of this region in both mitochondrial and chloroplastic import.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija

POVEZANOST RADA