Naslov
Low doses of gamma-rays can induce the expression of mdr gene

Autori
Osmak, Maja ; Miljanić, Saveta ; Kapitanović, Sanja

Izvornik
Mutation research (0027-5107) 324 (1994), 1-2; 35-41

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
gamma-irradiation ; drug resistance ; P-glycoprotein

Sažetak
We reported earlier that human cervical carcinoma HeLa cells exposed to 30 fractions of 0.5 Gy gamma-rays became resistant to cisplatin, methotrexate and vincristine, but retained the same sensitivity to gamma-rays and ultraviolet light. The aim of this study was to examine whether a small number of gamma-ray fractions, with a lower daily dose, may also change the sensitivity of preirradiated cells to different cytotoxic drugs. Using the modified MTT staining procedure, we found that cells preirradiated with 5 or 10 daily fractions of only 0.17 Gy gamma-rays did not alter their sensitivity to mitomycin, cisplatin, methotrexate, 5-fluorouracil, etoposide and doxorubicin. However, 10 fractions of gamma- rays induced resistance to vincristine and vinblastine. Our immunocytochemical experiments using monoclonal antibody JSB-1 show that the plasma membrane P-glycoprotein is involved in the induced resistance to Vinca alkaloids.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA