Functional tyrosine residue in the active center of human dipeptidyl peptidase III

Salopek-Sondi, Branka; Vukelić, Bojana; Špoljarić, Jasminka; Šimaga, Šumski; Vujaklija, Dušica; Makarević, Janja; Jajčanin, Nina; Abramić, Marija

doi:10.1515/BC.2008.021

Pregled bibliografske jedinice broj: 321653

Functional tyrosine residue in the active center of human dipeptidyl peptidase III

Salopek-Sondi, Branka; Vukelić, Bojana; Špoljarić, Jasminka; Šimaga, Šumski; Vujaklija, Dušica; Makarević, Janja; Jajčanin, Nina; Abramić, Marija

Functional tyrosine residue in the active center of human dipeptidyl peptidase III // Biological chemistry, 389 (2008), 2; 163-167 doi:10.1515/BC.2008.021 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 321653 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Functional tyrosine residue in the active center of human dipeptidyl peptidase III

Autori
Salopek-Sondi, Branka ; Vukelić, Bojana ; Špoljarić, Jasminka ; Šimaga, Šumski ; Vujaklija, Dušica ; Makarević, Janja ; Jajčanin, Nina ; Abramić, Marija

Izvornik
Biological chemistry (1431-6730) 389 (2008), 2; 163-167

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
aminopeptidase ; peptidase family M49 ; site-directed mutagenesis

Sažetak
Human dipeptidyl peptidase III (DPP III) is a member of the metallopeptidase family M49 with an implied role in the pain-modulatory system and endogenous defense against oxidative stress. Here, we report the heterologous expression of human DPP III and the site-directed mutagenesis results which demonstrate a functional role for Tyr318 at the active site of this enzyme. The substitution of Tyr318 to Phe decreased kcat by two orders of magnitude without altering the binding affinity of substrate, or of a competitive hydroxamate inhibitor designed to interact with S1 and S2 subsites. The results indicate that the conserved tyrosine could be involved in transition state stabilization during the catalytic action of M49 peptidases.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija

Napomena
DOI 10.1515/BC.2008.021

POVEZANOST RADA

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