Naslov
Pregnancy modulates the course of MCMV infection in balb/c mice
(Pregnancy modulates the course of mcmv infection in balb/c mice)

Autori
Tomac, Jelena ; Cekinović, Đurđica ; Arapović, Jurica ; Zafirova, Biljana ; Panić, Linda ; Bralić, Marina ; Pernjak Pugel, Ester ; Jonjić, Stipan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
2007 Annual meeting of the Croatian Immunological Society : Book of Abstracts / Rabatić, Sabina et al. - Zagreb : Hrvatsko imunološko društvo, 2007, 29-29

Skup
2007 Annual meeting of the Croatian Immunological Society

Mjesto i datum
Rovinj, Hrvatska, 19.10.2007. - 21.10.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
MCMV ovary pregnancy infection

Sažetak
Data from both human and animal studies clearly demonstrate that sex steroid hormones influence components of both innate and adaptive immunity, resulting in differences of immune responses between females and males. Moreover hormonal changes associated with pregnancy modify the severity of some infections and diseases. The aim of this study was to test whether the pregnancy influence the course of cytomegalovirus (CMV) infection in female BALB/c mice. Mice used in all experiments were treated with pregnant mare serum gonadotropin (PMGS) and human chorionic gonadotropin (hCG) to trigger ovulation and luteinization. Virgin animals as well as pregnant ones were intravenously infected with murine CMV (MCMV) and sacrificed each day from 1st to 10th day post infection. Viral titers were analyzed in different organs using standard plaque assay, while viral spread was examinated using immunohistochemistry. Our results showed higher susceptibility of pregnant mice to MCMV infection in all investigated organs. Moreover ovaries of pregnant mice showed delay in clearance of productive infection as compared to nonpregnant animals. We observed numerous clusters of infected cells situated in luteal bodies, whereas no cells positive for viral antigens were found within follicles. Our study provides a new insight in the pathogenesis of MCMV infection in female reproductive tissue as well as its dependency of sex steroid environment. These findings could bring the evidence of CMV infection in mechanisms of gonadal dysfunction.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA