Naslov
Adjuvant effects on induction of Th1/Th2-type of immune response specific for ovalbumin in mice immunised with novel Montanide ISA oil-based formulations

Autori
Habjanec, Lidija ; Halassy, Beata ; Tomašić, Jelka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Modern vaccines adjuvants formulations / - , 2007

Skup
Modern vaccines adjuvants formulations

Mjesto i datum
Dublin, Irska, 12.11.2007. - 14.11.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
oil-based adjuvants; ISA720; ISA206; Th1/Th2 immune response

Sažetak
The aim of the study was to investigate the influence of novel oil-based formulations on induction of Th1/Th2-type of immune response. Oil-based formulations tested were two commercial adjuvants, Montanide ISA720 and ISA206, and their novel formulations containing immunostimulating peptidoglycan monomer (ISA720PGM and ISA206PGM). IgG isotype profiles (IgG1/IgG2a ratio) and specific cytokine secretion (IFN-γ and IL-4) as specific markers of Th1/Th2-type of immune response were monitored in experimental mice using ovalbumin (OVA) as an antigen. We noticed differences in immune responses related to genetic background of the two mice strains used in the study, i.e. CBA and NIH/OlaHsd mice, respectively. In both mice strains, ISA720 formulations had similar effect to the positive control, CFA, and induced the switch towards Th1-type of immune response specific for OVA. However, ISA206 formulations were less effective in inducing the switch towards Th1 in CBA mice, while in NIH/OlaHsd mice improved the switch towards Th2-type of immune response.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Drvna tehnologija

POVEZANOST RADA