Naslov
Biological properties of 4-methyl-2, 7-diamino-5, 10-diphenyl-4, 9-diazapyrenium hydrogensulfate (ADAP)

Autori
Marczi, Saška ; Glavaš-Obrovac, Ljubica ; Belovari, Tatjana ; Stojković, Ranko ; Ivanković, Siniša ; Šerić, Vatroslav ; Piantanida, Ivo ; Žinić, Mladen

Izvornik
Cancer Chemotherapy and Pharmacology (0344-5704) 62 (2008), 4; 595-604

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
4; 9-diazapyrenium derivatives; cellular uptake; intracellular distribution; cytotoxicity in vitro; topoisomerase IIα; mice; toxicity in vivo

Sažetak
Objective 4-Metyl-2, 7-diamino-5, 10-diphenyl-4, 9-diazapyrenium hydrogensulfate (ADAP) is potential antitumor compound because of its DNA and RNA intercalating ability. In this study, cellular uptake, intracellular distribution as well as mechanism of action, antitumor activity in vitro and toxicity in vivo of ADAP were investigated. Methods Based on fluorescence properties of ADAP, its entry and distribution into live cells were analyzed by fluorescence microscopy. The in vitro antiproliferative activity was determined using MTT test. For screening of topoisomerase II-targeted effects of ADAP the cell-free assay and immunoband depletion assay were used. Expression of the genes c-mos, c-N-ras, c-Ki-ras, c-H-ras, p53 and caspase 3 in Caco-2 cells treated with ADAP was examined by RT-PCR. Toxicity in vivo was determined using C3HHf/Bu Zgr/Hr mice treated by a single or multiple doses of ADAP in concentration of 25 mg/kg. Results The ADAP in  M concentrations entered into MIAPaCa-2 cell’ s cytoplasm in five minutes and into nuclei in sixty minutes after administration. Intracellular distribution of ADAP depended on the period of treatment time. The ADAP (0.1 – 100 μ M) strongly inhibited growth of both mouse (FsaR, SCCVII) and human tumor cells (HeLa, Caco-2, HT-29, MIAPaCa-2, HBL, HEp-2, SW620, MCF-7) compared to its weak cytotoxicity on controls and normal cells (WI38). Results of both topoisomerase II assays showed that ADAP is not a topoisomerase II poison. Expression of investigated genes was dependent on the incubation time, except for p53 and c-H-ras. Morphological changes in tissues and organs of mice were not observed. Results of patohistological analysis have been confirmed by hematological and clinical-chemical analysis of blood of treated and non-treated animals. Conclusion The ADAP is a strongly bioactive compound with antitumor potential in vitro. The antitumor potential in vivo remains to be identified.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Temeljne medicinske znanosti

POVEZANOST RADA