Naslov
Peripheral blood lymphocytes subpopulations and immune response of chicks to inactivated Newcastle disease virus given by nebulization

Autori
Ciglar Grozdanić, Irena ; Šver, Lidija ; Čajavec, Stanislav ; Gottstein, Željko ; Židovec-Lepej, Snježana ; Mazija, Hrvoje

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
2007 Annual Meeting of the Croatian Immunological Society. Book of Abstract / - Zagreb : Hrvatsko imunološko društvo, 2007

Skup
2007 Annual Meeting of the Croatian Immunological Society

Mjesto i datum
Rovinj, Hrvatska, 19.10.2007. - 21.10.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
inactivated Newcastle disease virus; immune response; nebulization; lymphocytes

Sažetak
Newcastle disease (ND) is the most serious infectious disease of poultry causing substantial losses to the poultry industry. Vaccination programs against ND include the use of live vaccines administrated mainly mass by spray or aerosol, but vaccinal reaction often appears. Inactivated vaccines are applied for revaccination and require individual application. The present study was carried out to examine the possible induction of immune response following immunization of newly hatched chicks with inactivated La Sota NDV vaccine by ultrasonic nebulizer Sonovac&reg ; 095. Additionally, group of chicks was immunized with the same inactivated antigen in mixture with Parapoxvirus ovis as an immune response modifier (IRM) in order to test its immunostimulating activity. Immune response to inactivated NDV was estimated by HI test in week intervals up to 42nd day of age, and by survival after challenge infection. Nevertheless, inactivated antigen didn&#8217; t induce specific antibodies response, which could be detected by HI test, but chickens remained fully protected after challenges (70-100%). Additionally, the changes of leukocytes subpopulation in peripheral blood were analyzed by means of flow cytometry using specific monoclonal antibodies for chicken CD45, Ia, Bu-1 and IgA cell surface molecules. Significant increase (p<0.05) in B cells subpopulations (phenotypes Bu-1+, Bu-1+Ia+, Bu-1+IgA+) was observed 3 days post immunization in vaccinated groups. Further investigations should include the use of more effective immunomodulator and different (higher) dose of inactivated antigen. Results achieved indicate the possible use of inactivated NDV antigen in specific immunoprotection of newly hatched chicks.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Veterinarska medicina

POVEZANOST RADA