Pregled bibliografske jedinice broj: 310772
NUCLEAR DNA QUANTIFICATION OF BONE SAMPLES FROM DIFFERENT SOURCES: A COMPARISON BETWEEN REAL-TIME PCR, AGAROSE GEL ELECTROPHORESIS AND SPECTROPHOTOMETRY
NUCLEAR DNA QUANTIFICATION OF BONE SAMPLES FROM DIFFERENT SOURCES: A COMPARISON BETWEEN REAL-TIME PCR, AGAROSE GEL ELECTROPHORESIS AND SPECTROPHOTOMETRY // 5th ISABS Conference in Forensic genetics and Molecular Anthropology / Primorac, Dragan (ur.).
Zagreb: Studio Hrg, 2007. (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 310772 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
NUCLEAR DNA QUANTIFICATION OF BONE SAMPLES FROM DIFFERENT SOURCES: A COMPARISON BETWEEN REAL-TIME PCR, AGAROSE GEL ELECTROPHORESIS AND SPECTROPHOTOMETRY
Autori
Sutlović, Davorka ; Gugić, Dijana ; Definis-Gojanović, Marija ; Anđelinović, Šimun
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Skup
5th ISABS Conference in Forensic genetics and Molecular Anthropology
Mjesto i datum
Split, Hrvatska, 03.09.2007. - 07.09.2007
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
analitical methods; human DNA; quantification; bone samples
Sažetak
To comparate analytical methods to evaluate nuclear DNA concentrations of forensic and ancient bone samples. Three different methods were used: agarose gel electrophoresis, spectrophotometry and Quantitative Real-time PCR. QRT-PCR offers several advantages with respect to other compared methods. Twenty four DNA extracts from bone samples: eight DNA extracts from ancient bone samples (3 DNA samples retrieved from 10-50 years old bones and 5 DNA samples obtained from about 1300 years old bones recovered from an old church burrial sight), eight DNA were extraced from mass graves bones and eight were extracted from fresh bones. The quantification assay was performed in total volume of 25  L containing 2  L of DNA extract, Quantifiler human primer mix and Quantifiler PCR reaction mix with thermal cycling conditions according to the manufacture’ s protocols In the following trials total DNA was evaluated by gel electrophoresis (1.5% agarose) and ethidium bromide staining by UV iluminations (312 nm) of the gel . Absorbancies at 260 and 280 nm were measured by spectrophotometer. Highly sensitive methods for quantification of human DNA retrieved from forensic and ancient DNA samples are important because they ensure the consistency of low copy number DNA typing. RT-PCR offers several advantages with respect to other compared methods. In samples which contain high amounts of DNA all of the tested methods gave positive results with good agreement. All samples that possibly contain small amount of DNA or inhibitors therefore need to be quantitated by QRT-PCR as method of choice.
Izvorni jezik
Engleski
Znanstvena područja
Kemija, Biologija, Temeljne medicinske znanosti
POVEZANOST RADA
Projekti:
141-2160800-0333 - DNA analiza ranosrednjovjekovne populacije s područja južne Hrvatske
216-2160800-0655 - Metali u kosturima iz starohrvatskih grobova u Kliškoj i Bribirskoj županiji (Sutlović, Davorka, MZOS ) ( CroRIS)
216-2160800-0799 - Antropološka analiza kostura ranosrednjovjekovne populacije iz južne hrvatske (Definis-Gojanović, Marija, MZOS ) ( CroRIS)
Ustanove:
KBC Split,
Medicinski fakultet, Split
