Naslov
A one step solution for identification of the skeletal remains

Autori
Erceg, Ivana ; Anđelinović, Šimun ; Sutlović, Davorka ; Režić, Boja ; Ivkošić, Ante ; Primorac, Dragan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
THE SECOND EUROPEAN-AMERICAN INTENSIVE COURSE IN CLINICAL AND FORENSIC GENETICS - Abstract book / Primorac, Dragan - Zagreb, 2001

Skup
Second European – American intensive course in clinical and forensic genetics

Mjesto i datum
Dubrovnik, Hrvatska, 03.09.2001. - 14.09.2001

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
DNA isolation; IQ isolation system; AluQuant System; mass graves

Sažetak
Various forensic techniques have been applied in our laboratory in an effort to improve identification process of the victims found in several mass graves in Croatia and Bosnia and Herzegovina. In the majority of analyzed bones and teeth samples we observed the presence of minimal amount (pg) of degraded human DNA mixed with high amounts of microbial DNA. Analysis of hundred different samples may be time-consuming processes. Here we described a use of the new IQ DNA isolation system (Promega, USA), which is short and efficient method. Following the DNA isolation method all samples have been analyzed by AluQuant System and amplified with either Power Plex 16 System or AmpFlSTR ProfilerTM PCR Amplification Kit. Typing of PCR products was performed on ABI Prism 310 Genetic Analyzer.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Javno zdravstvo i zdravstvena zaštita

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