Naslov
Granulysin mediates long term cytolytic activity of the first trimester pregnancy decidual NK cells

Autori
Veljković, Danijela ; Laškarin, Gordana ; Rubeša, Željka ; Vlastelić, Ivan ; Piccinni, Marie-Pierre ; Saito, Shigeru ; Rukavina, Daniel

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstract book: 3rd EMBIC Summer School / Markert, Udo - Jena : Frierich Schiller Universitat, 2007, 51-51

Skup
3rd EMBIC Summer School

Mjesto i datum
Jena, Njemačka, 03.09.2007. - 07.09.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
granulysin; cytotoxicity; early pregnancy; K562

Sažetak
Problem:We investigated the granulysin mediated cytotoxicity of early pregnancy decidual CD56 positive (dCD56+) cells against NK-sensitive K562 cell line. Methods of study: CD56+ cells were positively selected in a magnetic field from a non-adherent decidual mononuclear cell fraction after 18-hrs culture at 37°C. Granulysin expression in unstimulated or K562 pulsed dCD56+ cells was analyzed using flow cytometry, immunohistochemistry or confocal microscopy. The cytotoxicity of CD56+ cells against K562 cells was investigated using PKH-26 red 2-hrs and 18-hrs cytotoxicity assay. Results: Approximately 90% of unstimulated decidual NK cells express granulysin in their cytoplasmatic granules located near the cell membrane. After the contact with K562 cells, the distribution of granulysin+ granules aquired more diffuse distribution. Although dCD56+ cells lyse K562 cells in 2-hrs and 18-hrs cytotoxicity test, the addition of anti-granulysin antibody decreased only 18-hrs cytotoxicity. Conclusion: Granulysin seems to be a long term cytolytic mediator employed by dCD56+ cells. Acknowledgement: The experiments are financed by Croatian Ministry of Science, grant No. 0620402-0376 and EMBIC project, European FP6 project No. 512040, NoE, LSHM-CT-2004-512040

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA