Naslov
Cytotoxicity of CD1a+ first trimester pregnancy decidual cells towards autologuos CD3+ cells

Autori
Rubeša, Željka ; Laškarin, Gordana, Redžović, Arnela, Veljković, Danijela, Haller, Herman, Allavena, Paola ; Mantovani, Alberto ; Rukavina, Daniel

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstract book: 3rd EMBIC Summer School / Markert, Udo - Jena : Friedrich Schiller Universitat, 2007, 45-45

Skup
3rd EMBIC Summer School

Mjesto i datum
Jena, Njemačka, 03.09.2007. - 07.09.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
cytotoxicity; pregnancy; decidual mononuclear cells; CD1a+ cells

Sažetak
Problem: Cytolytic potential of CD1a+ cells towards autologuos T cells of first trimester pregnancy decidua was investigated. Methods of study: Decidual mononuclear cells (DMC) were obtained by enzimatic digestion and gradient density centrifugation. Cytolytic mediators were analysed in CD1a+ cells using cell permeabilisation method. CD1a positive magnetic separation was performed from adherent DMC fraction whereas CD3+ cells were magnetically separated from CD56-CD83- DMC fraction using α -CD6 MicroBeads. Purified CD1a+ and T cells were co-cultured and short and long term cytolytic activity of CD1a+ cells towards CD3+ cells was tested using PKH-26 red assay. Results: Decidual CD1a+ cells highly express CD14 and CD56 molecules and are equipped with surface NK receptors as well as cytolytic mediators. CD1a+ cells show cytolytic activity against autologuos CD3+ cells. The percentage of CD3+ cells decreases after 3-day co-culture with CD1a+ cells. Conclusion: Decidual CD1a+ cells seem to be important for the regulation of T cells presence in early pregnancy decidua. Acnowledgement: The experiments are financed by Croatian Ministry of Science, No. 0620402-0376 and EMBIC project, European FP6 project No. 512040, NoE, LSHM-CT-2004-512040

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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