Naslov
Phenotypic and functional characteristic of the first trimester pregnancy decidual CD1a+ dendritic cells

Autori
Rubeša, Željka ; Laškarin, Gordana ; Redžovic, Arnela ; Veljković, Danijela ; Haller, Herman ; Allavena, Paola ; Mantovani, Alberto ; Rukavina, Daniel

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
American Journal of Reproductive Immunology / Beaman, Kenneth ; Rukavina, Daniel - Oxford : Wiley-Blackwell, 2007, 464-464

Skup
X International Congress of Reproductive Immunology

Mjesto i datum
Opatija, Hrvatska, 10.06.2007. - 14.06.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
dendritic cells; pregnancy; decidua

Sažetak
Problem: The study was aimed to analyse phenotype and functional properties of the first trimester normal human pregnancy decidual CD1a+ dendritic cells (DC). Method of study: Decidual mononuclear cells (DMC) are obtained by enzimatic digestion and gradient density centrifugation. The phenotype of CD1a+ DC (differentiation and maturation markers, as well as NK cytotoxicity receptors) was analysed immediately after the isolation and in both: adherent and non-adherent DMC fraction obtained after the 18-hour culture in vitro. In freshly isolated DMC, cell permeabilisation method with simultaneous surface CD1a labeling was used for cytokine and cytolytic mediator detection. Results: Decidual CD1a+ cells are lin-1 negative and show mostly myeloid, immature DC phenotype according relatively low CD83, CD80, CD86, HLA-DR and high CD14 molecule expression. There is no significant difference in the phenotype of CD1a+ DC between adherent and nonadherent fraction. CD1a+ cells from freshly isolated suspensions express mostly IL-15 and IL-18 cytokines, the percentage of IFN- + and TNF- + cells was approximately 2 times lower and the percentage of IL-10 producing cells was negligable. CD1a+ cells are equiped with short and long term cytolytic mediators, as well as surface NK cytotoxicity receptors. Conclusion: Mostly immature CD1a+ DC represent a source of pro-inflammatory cytokines and could be involved in cytotoxicity reactions in decidua according to their equipment with cytolytic armentarium. The experiments are partially financed by Croatian Ministry of Science Grant No. 0620402-0376 and EMBIC project, European FP6, NoE, No. 512040, LSHM-CT-2004-512040.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA