Naslov
Regulatory T and NKT cells in syngeneic pregnancy

Autori
Mrakovčić-Šutić, Ines ; Jakovac, Hrvoje ; Grebić, Damir ; Radošević-Stašić, Biserka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of abstracts / Sabina, Rabatić et al. - Zagreb : Hrvatsko imunološko društvo, 2007, 49-49.

Skup
2007 Annual Meeting of the Croatian Immunological Society

Mjesto i datum
Rovinj, Hrvatska, 19.10.2007. - 21.10.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
syngeneic pregnancy; NKT cells; regulatory T cells; peptidoglycan monomer

Sažetak
NKT cells and regulatory T cells (Treg) were claimed to be important players in the tolerance towards the fetus bearing alloantigens, having the pivotal role in triggering and inhibition of immune responses against self-antigens, as well as in regulation of innate immune response. They, however, might be related also with fetal invasion mechanisms at the maternal-fetal interface, with bacterial exposure, as well as with the systemic hormonal changes, induced by pregnancy, showing that they reflect more global changes of the maternal immune system. In an attempt to enlarge our previous findings showing that during syngeneic pregnancy in the liver accumulate cytotoxic self-reactive NKT cells, which usually cooperate with Treg cells, in this study we estimated the presence of CD4+CD25+Foxp3+ T cells in the liver, spleen and thymus of nonpregnant and pregnant C57/BL6 females matted with C57 BL/6 males. Furthermore, to examine the potential effects of bacterial toxins a group of pregnant mice was treated with peptidoglycan-monomer linked with zinc (PGM-Zn). Phenotypic analysis and cytotoxicity against syngeneic thymocytes and YAC-1 targets was made on the 16th day after detection of vaginal plugs. The data have shown that during syngeneic pregnancy in the maternal liver increases the proportion of CD4+CD25+Foxp3+, as well as CD3intermediate, NK1.1+ and IL-2Rb+ cells (NKT cells). Similar changes were induced by PGM-Zn, which additionally increased the Treg accumulation, particularly in the liver. The data point to interplay between the NKT and Treg cells, pointing to the liver as a place important for the activation of protective and tolerance-promoting mechanisms during the pregnancy.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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