Naslov
Heat shock protein 96 as a signal of stress and toxin-induced damages

Autori
Grebić, Damir ; Jakovac, Hrvoje ; Mrakovčić-Šutić, Ines ; Radošević-Stašić, Biserka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of abstracts / Sabina, Rabatić et al. - Zagreb : Hrvatsko imunološko društvo, 2007, 41-41

Skup
2007 Annual Meeting of the Croatian Immunological Society

Mjesto i datum
Rovinj, Hrvatska, 19.10.2007. - 21.10.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
gp96; stress; gasoline; brain; liver; kidney; lungs; NKT cells.

Sažetak
The endoplasmic reticulum (ER)- resident heat shock protein gp96 participates as molecular chaperon in the control of protein synthesis, degradation and transport through the various cellular compartments. Moreover, as carrier of antigenic peptides, gp96 has the ability to modulate the cellular immune response and activate the APC resulting in the production of pro-inflammatory cytokines and in up-regulation of costimulatory molecules.In an attempt to investigate some aspects of these events, in this study we investigated the effects of airborne pollutant gasoline vapor (GV) on gp96 expression in the liver, lungs, brain, kidney and lymphatic organs, estimating simultaneously the cytotoxicity of hepatic and splenic mononuclear lymphatic cells (MNLC) against NK sensitive target (YAC-1) and syngeneic thymocytes. Experiments were done on C57/BL6 mice, which were as a group of 6 animals for 1 h/day closed in a small metabolic chamber, containing soda lime for absorption of CO2 and ventilated with fresh air (stress control) or with air containing GV. Protocol was repeated for 10 days and then gp96 expression, phenotype of lymphoid cells and cytotoxicities of hepatic and splenic MNLC were detected by immunohistological staining and FACScan analysis. The findings were compared with data obtained in unstressed mice, living in the normal laboratory conditions. The data revealed that inhalation of GV upregulates the gp96 expression particularly in the hippocampus, lungs and kidneys, as well as in the liver, spleen and thymus. Simultaneously, in the liver accumulated NKT and NK cells and hepatic MNLC became more cytotoxic against the syngeneic thymocytes and less cytotoxic against the YAC-1 target (p<0.01). Stress of closure induced similar, but statistically less expressed changes, suggesting that during tissue damages and stress, gp96 might participate in activation of autoreactive clones of cells.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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