Naslov
Altered Plasma Levels of Granzyme K in Septic Patients: A Potential Early Marker in Sepsis

Autori
M. Ručević, L. Fast, G. Jay, S.M. Opal, Y.P. Lim.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
FASEB Journal 19(5) / - Bethesda (MD), 2005

Skup
FASEB Meeting Experimental Biology 2005 XXXV International Congress of Physiological Sciences

Mjesto i datum
San Diego (CA), Sjedinjene Američke Države, 02.04.2005. - 06.04.2005

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Granzyme K; sepsis; diagnostic marker
(Granzym K; sepsis; diagnostic marker)

Sažetak
Background: Granzymes (Grz) are serine proteases located in the granules of cytotoxic lymphocytes, known to play roles in target cell apoptosis and cytokine activation. Grz release appears to be modulated by abundant plasma serine proteases inhibitors. Inter-alpha inhibitor proteins (IaIp) have been recently identified as the physiological inhibitor of GrK. Our previous studies found a significant decrease of plasma IaIp in septic patients. Objective: To evaluate plasma GrK levels in healthy individuals and septic patients. Methods: An ELISA that measure GrK was developed by using anti-GrK polyclonal antibody against a peptide sequence unique for GrK that specifically binds to human native and recombinant GrK. The levels of GrK were measured in healthy controls (n=41) and two groups of septic patients: patients enrolled in the clinical trial (CT, n=25) and patients admitted in the Emergency Department (ED, n=16). The ED group represents the patients with the early stage of sepsis when the diagnosis was established at the time of admission. Results: While IaIp levels were significantly decreased in both septic groups, GrK levels were found significantly higher in ED patients and significantly lower in CT patients compared to HC (p=0.0001). Conclusion: The results suggest that systemic GrK level is altered during sepsis and may be involved in the pathogenesis of sepsis. GrK might be useful as an early indicator in patients developing sepsis.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti, Biotehnologija

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