Naslov
The effect of single dose of fumonisin B1 on oxidative stress, sphingolipid metabolism and DNA damage in rat kidney

Autori
Peraica, Maja ; Ljubanović, Danica ; Želježić, Davor ; Domijan, Ana-Marija

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the 44th Congress of the European Societies of Toxicology / Dekant, Wolfgang - Amsterdam : Elsevier, 2007

ISBN
172S S1-S240 (2007)

Skup
44th Congress of the European Societies of Toxicology

Mjesto i datum
Amsterdam, Nizozemska, 07.10.2007. - 10.10.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
mycotoxin; comet assay; glutathione; oxidative stress; histopathology; sphingolipids

Sažetak
Mycotoxin fumonisin B1 (FB1) is nephrotoxic, hepatotoxic and carcinogen in laboratory animals. It is already known that long exposure to this mycotoxin causes apoptosis and increases lipid peroxidation in kidney of experimental animals. The aim of this study was to check whether single dose and short exposure to this mycotoxin cause the same effects. In this study the groups (N=6) of adult male Wistar rats were given by gavage a single FB1 dose (5, 50, 500 mg/kg b.w.) and sacrificed four, 24 and 48 hours afterwards. The parameters of oxidative stress (glutathione-GSH and malondialdehyde-MDA), concentration of sphingolipids (sphinganine/sphingosine-Sa/So), DNA lesions (measured with comet assay) and histopathological changes were checked in kidney of treated and control animals. The lowest FB1 dose (5 mg/kg b.w) increased the Sa/So ratio after 4, 24 and 48 hours (2.53+0.52, 2.93+0.42 and 1.44+0.13, respectively) as compared to controls (0.62+0.10)(P<0.05), in contrast to GSH and MDA concentrations that were not changed even by the highest FB1 dose (500 mg/kg b.w). DNA lesions measured as comet tail length and tail intensity were FB1 dose- and time- dependent. The increase of tail intensity occurred at lower dose (5 mg/kg) than that of tail length (50 mg/kg). The application of higher FB1 doses caused the earlier appearance of DNA damage. Apoptotic cells and mitotic figures were found in cortical and outer stripe of medullar part of the kidney indicating the high potential of FB1 to produce degenerative and regenerative processes, probably due to changes in sphingolipid metabolism.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

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