Naslov
In Situ Amperometric Characterization of Liposome Suspensions with Concomitant Oxygen Reduction

Autori
Ivošević DeNardis, Nadica ; Žutić, Vera ; Svetličić, Vesna ; Frkanec, Ruža ; Tomašić, Jelka

Izvornik
Electroanalysis (1040-0397) 19 (2007), 23; 2444-2450

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
adhesion signals of single liposomes; amperometric detection; dropping mercury electrode; oxygen reduction; liposome suspension

Sažetak
Amperometry of oxygen reduction at the dropping mercury electrode (DME) was applied for in situ characterization of liposome suspensions in terms of concentration, level of polydispersity and potential range of adhesion. Liposomes prepared from egg-phosphatidylcholine/cholesterol/dicetylphosphate in the molar ratio of 7:5:1 were suspended in phosphate-buffered saline (PBS. Adhesion signals of single liposomes in air-saturated suspensions were detected in broad potential range (from-100 to -1200 mV vs. 0.1 M Ag/AgCl reference electrode) as transient enhacements of oxygen reduction.Measured concentration range in air-saturated suspensions was 10 6- 10 8 liposomes/L.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Temeljne medicinske znanosti

POVEZANOST RADA