Naslov
Acetylcholinesterase level and evaluation of genome by comet and micronucleus assay in carbofuran manufacturing workers

Autori
Radić, Božica ; Želježić, Davor ; Lucić Vrdoljak, Ana ; Fuchs, Radovan ; Berend, Suzana ; Kopjar, Nevenka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
International Congress of Toxicology : Abstracts / - Montréal : National Research Council Canada, 2007, 314-314

Skup
International Congress of Toxicology

Mjesto i datum
Montréal, Kanada, 15.07.2007. - 19.07.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
carbofuran; occupational exposure; AChE activity; micronucleus assay; centromere

Sažetak
Literature data on carbofuran genotoxicity in vitro and in vivo are very scarce. There are few papers indicating that occupational exposure to this AChE inhibiting insecticide might be connected to increased risk of developing non-Hodgkin’ s lymphoma and lung cancer. Other authors showed its genotoxicity in vitro. We used comet and CBMN micronucleus assay combined with centromere probes to evaluate genome damage in lymphocytes of workers employed in carbofuran production. Also, the level of AChE activity in blood and plasma was measured. Only few workers exhibited AChE activity below 85%. Comet assay parameters were slightly but significantly elevated compared to control subjects, especially the long-tailed nuclei ratio. We found poor correlation between AChE activity and comet assay parameters, but significant effect of smoking and alcohol intake on the latest. In binucleated lymphocytes of workers significantly increased number of micronuclei, nuclear buds, and nucleoplasmatic bridges was detected. Proportion of micronuclei with centromere, DAPI signal positive micronuclei was also elevated. Micronucleus assay parameters also appeared to be significantly influenced by duration of exposure to carbofuran. Together with published data on carbofuran’ s effect on health our results might indicate the need for further evaluations of its genotoxicity using a range of different cytogenetic techniques.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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