Naslov
Antibacterial activity of 0.5 isobaric bupivacain and 0.5 hyperbaric bupivacain in vitro

Autori
Mihaljević, Ljiljana ; Bedenić, Branka ; Mihaljević, Slobodan ; Šakić, Kata ; Radonić, Jelena ; Plavec, Davor

Izvornik
Periodicum Biologorum (0031-5362) 109 (2007), 3; 295-299

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
isobaric bupivacain; hyperbaric bupivacain; antibacterial activity

Sažetak
The purpose of the study was to investigate the antimicrobial effect of 0.5% bupivacaine in isobaric and hyperbaric form on Staphylococcus aureus, Escherichia coli, Enterococcus faecalis, ESBL positive Klebsiella pneumoniae, Pseudomonas aeruginosa and methicillin resistant Staphylococcus aureus (MRSA). The microorganisms were exposed to 0.5% isobaric and hyperbaric bupivacaine for 1-5 hours at 37 C. The overnight culture of the tested microorganisms was inoculated in 5 ml of anesthetic, incubated at 37 C, and the samples were taken every hour for determination of CFU/ml, diluted serially and seeded on Mueller-Hinton agar (E.faecalis on blood agar). Isobaric solution of 0.5% bupivacaine reduced the viable cells of E. coli and P. aeruginosa whereas hyperbaric solution of 0.5% bupivacaine reduced the viable cells of E. coli, S.aureus and ESBL positive K. pneumoniae. The hyperbaric solution (P<0.0001) reduced the E.coli count remarkably more then isobaric (P=0.0053). There was no influence of control solutions, neither the sterile 0.9% saline nor the sterile 8% glucose on the growth of any microorganism. Regarding to antimicrobial activity on tested strains, 0.5% hyperbaric bupivacaine has advantage over isobaric solution.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA