Naslov
Heat shock proteins and metallothionein expression in tissues of marine mussel Mytilus galloprovincialis as sensors of environmental pollution

Autori
Bulog, Aleksandar ; Jakovac, Hrvoje ; Grebić, Damir ; Mrakovčić-Šutić, Ines ; Mićović, Vladimir ; Radošević-Stašić, Biserka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Periodicum biologorum / Vitale, B. - Zagreb : Hrvatsko prirodoslovno društvo, 2007, 155-155

Skup
5th Croatian Congress of Pharmacology and 2th Congress of Croatian Physiological Society

Mjesto i datum
Osijek, Hrvatska, 19.09.2007. - 22.09.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
metallothionein; heat shock protein 70; marine mussels; environmental pollution

Sažetak
Introduction: Exposure of cells to wide variety of stressors, such as heat, heavy metals, organic poison, injuries, hypoxia etc alters the tertiary structure of proteins and leads in cells to general stress response, which consists in transient enhancement of the expression of cytoprotective proteins- heat shock proteins (HSP) and metallothioneins (MT), which act as molecular chaperons or as regulators of heavy-metal homeostasis, respectively. Since both families, as evolutionary conserved proteins are distributed also in animals, in this study the expression of HPS 70 and MTs I/II have been evaluated in tissues of marine mussel Mytilus galloprovincialis collected along the coast of the Kvarnerian bay in the zones, which are differently exposed to environmental pollution (industrial, urban, peri-urban and turistic area), as well as in mussels exposed to heat or to CdCl2 and ZnCl2 in vitro. Material and methods: The level of HSP 70 and MTs expression were detected in mussel gills and the digestive gland, using Western blot analysis and immunohistochemistry. Results: The data have shown that there are significant differences in HSP 70 and MTs expression in mussels collected at zones with different pollution, as well as that they differently react on stressors (heat shock and toxic metals) used in vitro. Conclusion: The data suggests that HPS and MTs may be useful biomarkers of environmental pollutions.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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