Naslov
Microtubule Impairment and Micronucleus Prevalence in Cultured Cells Following Mobile-Phone Frequency Irradiation

Autori
Pavičić, Ivan ; Trošić, Ivančica

Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni

Izvornik
International EMF Conference 2007 : Book of Proceedings / Hg, K.H. - Kuala Lumpur : University Malaya, 2007, 101-104

Skup
International EMF Conference 2007 : Electromagnetic Fields, Bioeffects Research, Medical Applications, and Standards Harmonization

Mjesto i datum
Kuala Lumpur, Malezija, 04.06.2007. - 08.06.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
mobile-phone radiation; microtubule proteins; micronuclei

Sažetak
It is known that radiofrequency radiation (RF) may affect biological systems as a result of interference with polar macromolecular cell structures. This study was aimed to evaluate the extent of microtubule disturbance and micronucleus appearance in continuous cell culture of hamster&#8217; s lung fibroblasts (V79) exposed to the mobile phone frequency of 935 MHz. Irradiation of cell samples was performed within the RF exposure set-up, which is Hewlett Packard signal generator and Gigahertz Transversal Electromagnetic Mode cell (GTEM-cell). Physical properties of electromagnetic surroundings in the GTEM cell was determined to be 8.2&#177; 0.3 V/cm, 0.017 W/m2 and averaged specific absorption rate 0.12 W/kg (SAR). Cell samples were exposed to the continuous waves of 935 MHz frequency for 1, 2 and 3 hours. Besides the cell samples intending to be irradiated experimental procedure included unexposed- and positive controls. The microtubule proteins were determined by means of specific immunocytochemical methodology. After exposure the cell chamber slides were prepared and analyzed by means of fluorescence microscopy. Micronuclei in binucleated and multinucleated interphase cells were determined as a result of microscope analysis of Giemsa stained cell smears twenty four hours after treatment. Compared to the controls, the microtubule structure in the exposed cells was significantly altered after 3 hours of irradiation (p<0.05). Moreover, micronucleus occurrence significantly increased for the same time of radiation, i.e., three hours of exposure (p<0.05). It can be concluded that under the applied conditions of exposure, mobile-phone radiation (935 MHz) affects microtubule proteins which is correlated with increased frequency of micronuclei appearance in irradiated cells

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA