Naslov
G-protein coupled receptors for succinate (GPR91)and alpha ketoglutarate (GPR99) are differently localized in the human and mouse nephron

Autori
Micek, Vedran ; Brzica, Hrvoje ; Balen, Daniela ; Ljubojević, Marija ; Breljak, Davorka ; Anzai, Naohiko ; Koepsell, Hermann ; Sabolić, Ivan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Periodicum biologorum, Final Programme and Abstract Book / Banfić, H ; Boban, M ; Francetić, I ; Klarica, M ; Muck-šeler, D ; Pivac, N ; Sabolić, I ; Tvrdeić, A ; Župan, G - Zagreb : Hrvatsko prirodoslovno društvo, 2007, Vol. 109, 151-151

Skup
5th CROATIAN CONGRESS OF PHARMACOLOGY AND 2nd CONGRESS OF CROATIAN PHYSIOLOGICAL SOCIETY

Mjesto i datum
Osijek, Hrvatska, 19.09.2007. - 22.09.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
receptors; succinate; alpha ketoglutarate; human; mouse

Sažetak
It has been suggested that the G-protein-coupled receptors GPR91 (receptor for succinate) and GPR99 (receptor for  -ketoglutarate), that are localized in the mammalian nephron, may sense local levels of succinate and  -ketoglutarate, respectively, and then function as controlers of blood pressure via affecting the activity of renin-angiotensin system. In the mouse kidney, mRNA for these receptors was highly expressed and localized to the proximal (GPR91) and distal (GPR99) tubules, but the exact localization of receptor proteins along the mammalian nephron is not known. In order to screen localization of these receptors in the human kidney, in this study we used several commercial polyclonal antibodies against the peptide sequences specific for the human GPR91 and GPR99 and tested their efficiency in detecting the respective proteins by Western blotting (WB) of total cell membranes isolated from the tissue homogenates, and by immunofluorescence cytochemistry (IC) in tissue cryosections. In WB studies, the optimal antibodies from both groups labeled a single protein band of ~90 kDa (reducing conditions at 37 oC) that dropped to ~50 kDa at higher heating temperatures (reducing conditions at 65-95 oC). In IC studies, the optimal GPR91-antibody stained: a) the basolateral domain of thick ascending limbs of Henle, which were distinguished from the high affinity Na+-glucose cotransporter (SGLT1)-positive proximal tubule S3 segments, and b) intercalated cells in the inner medullary collecting duct, where it colocalized with the vacuolar H+-ATPase. The GPR99-antibodies stained only the brush-border of cortical proximal tubules. In conclusion, GPR91 and GPR99 proteins exhibit specific localization in the human nephron which is different from that in the mouse nephron, and thus indicate possible different, species-specific function(s) of these receptors.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA