Naslov
Peroxisome proliferator-activated receptor gamma (PPARG) gene polymorphism in coronary artery disease patients and myocardial infarction survivors

Autori
Ferenčak, Goran ; Pašalić, Daria ; Gršković, Branka ; Marinković, Natalija ; Stavljenić Rukavina, Ana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Clinical Chemistry and Laboratory Medicine / Gerard Siest - Amsterdam : Walter de Gruyter, 2007

Skup
EUROMEDLAB Amsterdam 2007 17th IFCC-FESCC European Congress of Clinical Chemistry and Laboratory Medicine 60th National Congress of the Netherlands Society for Clinical Chemistry and Laboratory

Mjesto i datum
Amsterdam, Nizozemska, 03.06.2007. - 07.06.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Proliferator-activated receptor gamma (PPARG); PPARG gene; coronary artery disease; myocardial infarction

Sažetak
Background. Proliferator-activated receptor gamma (PPARG) regulates adipocytes differentiation, glucose homeostasis and long-term lipid storage. The detection of PPARG in lesion macrophages, coupled with its identification as the molecular target of antidiabetic agents, has raised significant interest in the potential role of this receptor in coronary artery disease (CAD) and myocardial infarction (MI). Methods. A new fluorescence resonance energy transfer (FRET) genotyping assay for Pro12Ala polymorphism in PPARG gene was developed and used to genotype subjects with or without angiographically documented CAD and MI survivors. Serum glucose and lipid parameters were measured by standard enzymatic methods. Results. Genotype frequencies were in Hardy-Weinberg equilibrium. Absolute frequencies of PPARG Pro12Ala gene variants did not differ significantly between CAD+ and CAD- subjects. Pro/Pro, Pro/Ala and Ala/Ala genotypes were 491, 174 and 14 in CAD+ group, and 241, 70 and 4 in CAD- group, respectively. However, they differed significantly between subjects with (MI+) and without (MI-) history of MI. Pro/Pro, Pro/Ala and Ala/Ala genotypes were 235, 103 and 5 in MI+ group, and 497, 140 and 13 in MI- group, respectively. In a logistic regression, assuming the recessive mode of inheritance and Pro/Pro as a reference genotype, odds ratio for MI after correction for gender was 1.52 (95% confidence interval 1.13 to 2.03), p=0.0055. Serum levels of glucose and lipid parameters did not differ significantly between the studied groups. Conclusions. PPARG gene variants may confer increased risk for MI. Higher frequency of rare 12Ala allele in MI survivors indicates a protective role for Pro12 allele.

Izvorni jezik
Engleski

Znanstvena područja
Javno zdravstvo i zdravstvena zaštita

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