Naslov
Enhancement by PL 14736 of granulation and collagen organization in healing wounds and the potential role of egr-1 expression

Autori
Ivetić Tkalčević, Vanesa ; Čužić, Snježana ; Brajša, Karmen ; Mildner, Boris ; Bokulić, Ana ; Šitum, Kristina ; Perović, Daniela ; Glojnarić, Ines ; Michael, J, Parnham

Izvornik
European Journal of Pharmacology (0014-2999) 570 (2007); 212-221

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
collagen ; egr-1 expression ; granulation tissue ; PDGF-BB ; PL 14736 ; wound healing

Sažetak
Apart from becaplermin (recombinant human platelet-derived growth factor homodimer of B chains, PDGF-BB), for the treatment of lower extremity diabetic ulcers, few agents are available for pharmacological stimulation of wound healing. We have compared the mechanism of action of the potential wound healing agent, PL 14736 (G E P P P G K P A D D A G L V), with that of PDGF-BB on granulation tissue formation following sponge-implantation in the normoglycemic rat and in healing full-thickness excisional wounds in db/db genetically diabetic mice. Expression of the immediate response gene, early growth response gene-1 (egr-1) was studied in Caco-2 cells in vitro. While PDGF-BB and PL 14736 had similar selectivity for stimulation of granulation tissue in both sponge granuloma and in healing wounds in db/db mice, PL 14736 was more active in stimulating early collagen organization. It also stimulated expression of egr-1 and its repressor nab2 in non-differentiated Caco-2 cells more rapidly than PDGF-BB. EGR-1 induces cytokine and growth factor generation and early extracellular matrix (collagen) formation, offering an explanation for the beneficial effects of PL 14736 on wound healing.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA